The molecular basis of neurotrophic keratitis

Harrison D Cavanagh, A. M. Colley

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Endogenous proliferation of corneal epithelial cells is regulated by a bidirectional control process characterized by an adrenergic, cAMP-dependent 'off', and a cholinergic, muscarinic cGMP-dependent 'on' response. The adrenergic receptor(s) are located in the plasma membrane (microsomal fraction), whereas the novel feature of the system is a cholinergic receptor specific for acetylcholine (ACH) located in the nuclear membrane. Exogenous substances which raise intracellular cAMP levels such as isoproterenol or PGE1, shut off epithelial mitosis; and, carbamylcholine or ACH raise intranuclear cGMP levels and increase mitosis by specific, regulatory stimulation of RNA-polymerase II activity. We believe that this regulatory system explains the transitory mitotic suppression induced by superficial corneal wounding (interruption of adrenergic fibres, 'chalone-effect'); and the marked, permanent depression of epithelial mitosis associated with decreased intracellular ACH levels which are produced by total corneal denervation, and which results in neurotrophic keratitis.

Original languageEnglish (US)
Pages (from-to)115-134
Number of pages20
JournalActa Ophthalmologica
Volume67
Issue numberSUPPL. 192
StatePublished - 1989

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Keratitis
Mitosis
Acetylcholine
Cholinergic Agents
Chalones
Adrenergic Fibers
RNA Polymerase II
Alprostadil
Nuclear Envelope
Carbachol
Cholinergic Receptors
Denervation
Isoproterenol
Adrenergic Agents
Adrenergic Receptors
Epithelial Cells
Cell Membrane

ASJC Scopus subject areas

  • Ophthalmology
  • Medicine(all)

Cite this

Cavanagh, H. D., & Colley, A. M. (1989). The molecular basis of neurotrophic keratitis. Acta Ophthalmologica, 67(SUPPL. 192), 115-134.

The molecular basis of neurotrophic keratitis. / Cavanagh, Harrison D; Colley, A. M.

In: Acta Ophthalmologica, Vol. 67, No. SUPPL. 192, 1989, p. 115-134.

Research output: Contribution to journalArticle

Cavanagh, HD & Colley, AM 1989, 'The molecular basis of neurotrophic keratitis', Acta Ophthalmologica, vol. 67, no. SUPPL. 192, pp. 115-134.
Cavanagh, Harrison D ; Colley, A. M. / The molecular basis of neurotrophic keratitis. In: Acta Ophthalmologica. 1989 ; Vol. 67, No. SUPPL. 192. pp. 115-134.
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