The Molecular Chaperone Hsp90 Is Required for Cell Cycle Exit in Drosophila melanogaster

Jennifer L. Bandura, Huaqi Jiang, Derek W. Nickerson, Bruce A. Edgar

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The coordination of cell proliferation and differentiation is crucial for proper development. In particular, robust mechanisms exist to ensure that cells permanently exit the cell cycle upon terminal differentiation, and these include restraining the activities of both the E2F/DP transcription factor and Cyclin/Cdk kinases. However, the full complement of mechanisms necessary to restrain E2F/DP and Cyclin/Cdk activities in differentiating cells are not known. Here, we have performed a genetic screen in Drosophila melanogaster, designed to identify genes required for cell cycle exit. This screen utilized a PCNA-miniwhite+ reporter that is highly E2F-responsive and results in a darker red eye color when crossed into genetic backgrounds that delay cell cycle exit. Mutation of Hsp83, the Drosophila homolog of mammalian Hsp90, results in increased E2F-dependent transcription and ectopic cell proliferation in pupal tissues at a time when neighboring wild-type cells are postmitotic. Further, these Hsp83 mutant cells have increased Cyclin/Cdk activity and accumulate proteins normally targeted for proteolysis by the anaphase-promoting complex/cyclosome (APC/C), suggesting that APC/C function is inhibited. Indeed, reducing the gene dosage of an inhibitor of Cdh1/Fzr, an activating subunit of the APC/C that is required for timely cell cycle exit, can genetically suppress the Hsp83 cell cycle exit phenotype. Based on these data, we propose that Cdh1/Fzr is a client protein of Hsp83. Our results reveal that Hsp83 plays a heretofore unappreciated role in promoting APC/C function during cell cycle exit and suggest a mechanism by which Hsp90 inhibition could promote genomic instability and carcinogenesis.

Original languageEnglish (US)
Article numbere1003835
JournalPLoS Genetics
Volume9
Issue number9
DOIs
StatePublished - Sep 2013

Fingerprint

molecular chaperones
Molecular Chaperones
Anaphase-Promoting Complex-Cyclosome
Drosophila melanogaster
cell cycle
Cell Cycle
Cyclins
protein
gene
cyclins
phenotype
inhibitor
mutation
genomics
Cell Proliferation
Eye Color
E2F Transcription Factors
cell proliferation
cdc Genes
Gene Dosage

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

The Molecular Chaperone Hsp90 Is Required for Cell Cycle Exit in Drosophila melanogaster. / Bandura, Jennifer L.; Jiang, Huaqi; Nickerson, Derek W.; Edgar, Bruce A.

In: PLoS Genetics, Vol. 9, No. 9, e1003835, 09.2013.

Research output: Contribution to journalArticle

Bandura, Jennifer L. ; Jiang, Huaqi ; Nickerson, Derek W. ; Edgar, Bruce A. / The Molecular Chaperone Hsp90 Is Required for Cell Cycle Exit in Drosophila melanogaster. In: PLoS Genetics. 2013 ; Vol. 9, No. 9.
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