The molecular forms of immunoreactive glucagon secreted by the isolated, perfused dog pancreas

J. Michael Conlon, Eli Ipp, Roger H Unger

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The immunoreactive glucagon (IRG) in the plasma-free effluent of the arginine stimulated isolated dog pancreas was purified by immunoaffinity chromatography and characterized with respect to molecular weight. Only a 3500 dalton component was secreted from the pancreas during the first four minutes of stimulation but immunoreactive material having a molecular weight of between 150,000 and 200,000 was isolated from the secretions after prolonged stimulation. This component (which corresponds in size to the incompletely characterized "big plasma glucagon") was dissociated to a 3500 dalton component and nonimmunoreactive material by 6 M guanadinium chloride. Components of molecular weight 9000 and 2000, which are found in plasma, and components with the immunological properties of gut GLI, were not identified in the pancreatic secretions.

Original languageEnglish (US)
Pages (from-to)1655-1658
Number of pages4
JournalLife Sciences
Volume23
Issue number16
DOIs
StatePublished - Oct 23 1978

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Glucagon
Pancreas
Molecular Weight
Molecular weight
Dogs
Plasmas
Chromatography
Arginine
Chlorides
Effluents

ASJC Scopus subject areas

  • Pharmacology

Cite this

The molecular forms of immunoreactive glucagon secreted by the isolated, perfused dog pancreas. / Michael Conlon, J.; Ipp, Eli; Unger, Roger H.

In: Life Sciences, Vol. 23, No. 16, 23.10.1978, p. 1655-1658.

Research output: Contribution to journalArticle

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