The molecular forms of immunoreactive glucagon secreted by the isolated, perfused dog pancreas

J. Michael Conlon; Eli Ipp; Roger H Unger

doi:10.1016/0024-3205(78)90462-9

The molecular forms of immunoreactive glucagon secreted by the isolated, perfused dog pancreas

J. Michael Conlon, Eli Ipp, Roger H Unger

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6 Scopus citations

Abstract

The immunoreactive glucagon (IRG) in the plasma-free effluent of the arginine stimulated isolated dog pancreas was purified by immunoaffinity chromatography and characterized with respect to molecular weight. Only a 3500 dalton component was secreted from the pancreas during the first four minutes of stimulation but immunoreactive material having a molecular weight of between 150,000 and 200,000 was isolated from the secretions after prolonged stimulation. This component (which corresponds in size to the incompletely characterized "big plasma glucagon") was dissociated to a 3500 dalton component and nonimmunoreactive material by 6 M guanadinium chloride. Components of molecular weight 9000 and 2000, which are found in plasma, and components with the immunological properties of gut GLI, were not identified in the pancreatic secretions.

Original language	English (US)
Pages (from-to)	1655-1658
Number of pages	4
Journal	Life Sciences
Volume	23
Issue number	16
DOIs	https://doi.org/10.1016/0024-3205(78)90462-9
State	Published - Oct 23 1978

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
Pharmacology, Toxicology and Pharmaceutics(all)

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10.1016/0024-3205(78)90462-9

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title = "The molecular forms of immunoreactive glucagon secreted by the isolated, perfused dog pancreas",

abstract = "The immunoreactive glucagon (IRG) in the plasma-free effluent of the arginine stimulated isolated dog pancreas was purified by immunoaffinity chromatography and characterized with respect to molecular weight. Only a 3500 dalton component was secreted from the pancreas during the first four minutes of stimulation but immunoreactive material having a molecular weight of between 150,000 and 200,000 was isolated from the secretions after prolonged stimulation. This component (which corresponds in size to the incompletely characterized {"}big plasma glucagon{"}) was dissociated to a 3500 dalton component and nonimmunoreactive material by 6 M guanadinium chloride. Components of molecular weight 9000 and 2000, which are found in plasma, and components with the immunological properties of gut GLI, were not identified in the pancreatic secretions.",

author = "{Michael Conlon}, J. and Eli Ipp and Unger, {Roger H}",

note = "Funding Information: This work was supported by VA Institutional Research Support Grant 549-8000-O1 ; National Tnstitutes of Health Grant AM 02700-16 and Contract NO1-AM-62219 ; and The Salk Institute - Texas Research Foundation. J. Michael Conlon is the recipient of a Medical Research Council (London) Traveling Fellowship.",

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doi = "10.1016/0024-3205(78)90462-9",

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T1 - The molecular forms of immunoreactive glucagon secreted by the isolated, perfused dog pancreas

AU - Michael Conlon, J.

AU - Ipp, Eli

AU - Unger, Roger H

N1 - Funding Information: This work was supported by VA Institutional Research Support Grant 549-8000-O1 ; National Tnstitutes of Health Grant AM 02700-16 and Contract NO1-AM-62219 ; and The Salk Institute - Texas Research Foundation. J. Michael Conlon is the recipient of a Medical Research Council (London) Traveling Fellowship.

PY - 1978/10/23

Y1 - 1978/10/23

N2 - The immunoreactive glucagon (IRG) in the plasma-free effluent of the arginine stimulated isolated dog pancreas was purified by immunoaffinity chromatography and characterized with respect to molecular weight. Only a 3500 dalton component was secreted from the pancreas during the first four minutes of stimulation but immunoreactive material having a molecular weight of between 150,000 and 200,000 was isolated from the secretions after prolonged stimulation. This component (which corresponds in size to the incompletely characterized "big plasma glucagon") was dissociated to a 3500 dalton component and nonimmunoreactive material by 6 M guanadinium chloride. Components of molecular weight 9000 and 2000, which are found in plasma, and components with the immunological properties of gut GLI, were not identified in the pancreatic secretions.

AB - The immunoreactive glucagon (IRG) in the plasma-free effluent of the arginine stimulated isolated dog pancreas was purified by immunoaffinity chromatography and characterized with respect to molecular weight. Only a 3500 dalton component was secreted from the pancreas during the first four minutes of stimulation but immunoreactive material having a molecular weight of between 150,000 and 200,000 was isolated from the secretions after prolonged stimulation. This component (which corresponds in size to the incompletely characterized "big plasma glucagon") was dissociated to a 3500 dalton component and nonimmunoreactive material by 6 M guanadinium chloride. Components of molecular weight 9000 and 2000, which are found in plasma, and components with the immunological properties of gut GLI, were not identified in the pancreatic secretions.

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The molecular forms of immunoreactive glucagon secreted by the isolated, perfused dog pancreas

Abstract

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