The mouse Clock locus: Sequence and comparative analysis of 204 kb from mouse chromosome 5

L. D. Wilsbacher, A. M. Sangoram, M. P. Antoch, J. S. Takahashi

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The Clock gene encodes a basic helix-loop-helix (bHLH)-PAS transcription factor that regulates circadian rhythms in mice. We previously cloned Clock in mouse and human using a battery of behavioral and molecular techniques, including shotgun sequencing of two bacterial artificial chromosome (BAC) clones. Here we report the finished sequence of a 204-kb region from mouse chromosome 5. This region contains the complete loci for the Clock and Tpardl (pFT27) genes, as well as the 3′ partial locus of the Neuromedin U gene; sequence analysis also suggests the presence of two previously unidentified genes. In addition, we provide a comparative genomic sequence analysis with the syntenic region from human chromosome 4. Finally, a new BAC transgenic line indicates that the genomic region that is sufficient for rescue of the Clock mutant phenotype is no greater than 120 kb and tightly flanks the 3′ end of the Clock gene.

Original languageEnglish (US)
Pages (from-to)1928-1940
Number of pages13
JournalGenome Research
Volume10
Issue number12
DOIs
StatePublished - Dec 1 2000

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'The mouse Clock locus: Sequence and comparative analysis of 204 kb from mouse chromosome 5'. Together they form a unique fingerprint.

  • Cite this