TY - JOUR
T1 - The mouse major histocompatibility complex
T2 - Some assembly required
AU - Amadou, Claire
AU - Kumánovics, Attila
AU - Jones, Elsy P.
AU - Lambracht-Washington, Doris
AU - Yoshino, Masayasu
AU - Lindahl, Kirsten Fischer
PY - 1999
Y1 - 1999
N2 - We have assembled a contig of 81 yeast artificial chromosome clones that spans 8 Mb and contains the entire major histocompatibility complex (Mhc) from mouse strain C57BL/6 (H2b), and we are in the process of assembling an Mhc contig of bacterial artificial chromosome (BAC) clones from strain 129 (H2(bc)), which differs from C57BL/6 in the H2-Q and H2-T regions. The current BAC contig extends from Tapasin to D17Leh89 with gaps in the class II, H2-Q, and distal H2-M regions. Only four BAC clones were required to link the class I genes of the H2-Q and H2-T regions, and no new class I gene was found in the previous gap. The proximal 1 Mb of the H2-M region has been analyzed in detail and is ready for sequencing; it includes 21 class I genes or fragments, at least 14 olfactory receptor-like genes, and a number of non-class I genes that clearly establish a conserved synteny with the class I regions of the human and rat Mhc.
AB - We have assembled a contig of 81 yeast artificial chromosome clones that spans 8 Mb and contains the entire major histocompatibility complex (Mhc) from mouse strain C57BL/6 (H2b), and we are in the process of assembling an Mhc contig of bacterial artificial chromosome (BAC) clones from strain 129 (H2(bc)), which differs from C57BL/6 in the H2-Q and H2-T regions. The current BAC contig extends from Tapasin to D17Leh89 with gaps in the class II, H2-Q, and distal H2-M regions. Only four BAC clones were required to link the class I genes of the H2-Q and H2-T regions, and no new class I gene was found in the previous gap. The proximal 1 Mb of the H2-M region has been analyzed in detail and is ready for sequencing; it includes 21 class I genes or fragments, at least 14 olfactory receptor-like genes, and a number of non-class I genes that clearly establish a conserved synteny with the class I regions of the human and rat Mhc.
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U2 - 10.1111/j.1600-065X.1999.tb01394.x
DO - 10.1111/j.1600-065X.1999.tb01394.x
M3 - Review article
C2 - 10319263
AN - SCOPUS:0032952989
SN - 0105-2896
VL - 167
SP - 211
EP - 221
JO - Immunological Reviews
JF - Immunological Reviews
ER -