The murine B cell response to TNP-polyacrylamide beads: The relationship between the epitope density of the antigen and the requirements for T cell help and surface IgD

E. Pure, E. S. Vitetta

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Spleen cells were cultured with high or low epitope TNP-polyacrylamide beads (TNP-PAB) in order to investigate the effect of epitope density on the requirements for T cell help and surface IgD on responding B cells. The response to low epitope density TNP-PAB was abolished by treatment witn anti-Thy-1.2 and complement, whereas approximately 50% of the response to high epitope density TNP-PAB was retained after similar treatment. Thus, an increase in epitope density resulted in a decreased requirement for T cell help. An increase in epitope density was also associated with a decreased requirement for interaction of antigen and surface IgD as determined by 'blocking' studies with anti-δ; further, the majority of the T-independent portion of this response was not blocked by anti-δ antibodies. This finding indicates that the T-independent portion of the anti-TNP response does not require interaction of antigen with surface IgD on B cells. These results are discussed in terms of differential cross-linking of IgM and IgD receptors on B cells by multivalent antigens.

Original languageEnglish (US)
Pages (from-to)420-427
Number of pages8
JournalJournal of Immunology
Volume125
Issue number1
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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