The murine liver-specific nonclassical MHC class I molecule Q10 binds a classical peptide repertoire

Francesca Zappacosta, Piotr Tabaczewski, Kenneth C. Parker, John E. Coligan, Iwona Stroynowski

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Abstract

The biological properties of the nonclassical class I MHC molecules secreted into blood and tissue fluids are not currently understood. To address this issue, we studied the murine Q10 molecule, one of the most abundant, soluble class Ib molecules. Mass spectrometry analyses of hybrid Q10 polypeptides revealed that α1α2 domains of Q10 associate with 8-9 long peptides similar to the classical class I MHC ligands. Several of the sequenced peptides matched intracellularly synthesized murine proteins. This finding and the observation that the Q10 hybrid assembly is TAP2-dependent supports the notion that Q10 groove is loaded by the classical class I Ag presentation pathway. Peptides eluted from Q10 displayed a binding motif typical of H-2K, D, and L ligands. They carried conserved residues at P2 (Gly), P6 (Leu), and Pω (Phe/Leu). The role of these residues as anchors/auxiliary anchors was confirmed by Ala substitution experiments. The Q10 peptide repertoire was heterogeneous, with 75% of the groove occupied by a multitude of diverse peptides; however, 25% of the molecules bound a single peptide identical to a region of a TCR V β-chain. Since this peptide did not display enhanced binding affinity for Q10 nor does its origin and sequence suggest that it is functionally significant, we propose that the nonclassical class I groove of Q10 resembles H-2K, D, and L grooves more than the highly specialized clefts of nonclassical class I Ags such as Qa-1, HLA-E, and M3.

Original languageEnglish (US)
Pages (from-to)1906-1915
Number of pages10
JournalJournal of Immunology
Volume164
Issue number4
StatePublished - Feb 15 2000

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Peptides
Liver
Ligands
Mass Spectrometry
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Zappacosta, F., Tabaczewski, P., Parker, K. C., Coligan, J. E., & Stroynowski, I. (2000). The murine liver-specific nonclassical MHC class I molecule Q10 binds a classical peptide repertoire. Journal of Immunology, 164(4), 1906-1915.

The murine liver-specific nonclassical MHC class I molecule Q10 binds a classical peptide repertoire. / Zappacosta, Francesca; Tabaczewski, Piotr; Parker, Kenneth C.; Coligan, John E.; Stroynowski, Iwona.

In: Journal of Immunology, Vol. 164, No. 4, 15.02.2000, p. 1906-1915.

Research output: Contribution to journalArticle

Zappacosta, F, Tabaczewski, P, Parker, KC, Coligan, JE & Stroynowski, I 2000, 'The murine liver-specific nonclassical MHC class I molecule Q10 binds a classical peptide repertoire', Journal of Immunology, vol. 164, no. 4, pp. 1906-1915.
Zappacosta, Francesca ; Tabaczewski, Piotr ; Parker, Kenneth C. ; Coligan, John E. ; Stroynowski, Iwona. / The murine liver-specific nonclassical MHC class I molecule Q10 binds a classical peptide repertoire. In: Journal of Immunology. 2000 ; Vol. 164, No. 4. pp. 1906-1915.
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