TY - JOUR
T1 - The myogenic regulatory factor MRF4 represses the cardiac α-actin promoter through a negative-acting N-terminal protein domain
AU - Moss, Jennifer Barnett
AU - Olson, Eric N.
AU - Schwartz, Robert J.
PY - 1996
Y1 - 1996
N2 - Cardiac α-actin is activated early during the development of embryonic skeletal muscle and cardiac myocytes. The gene product remains highly expressed in adult striated cardiac muscle yet is dramatically reduced in skeletal muscle. Activation and repression of cardiac α-actin gene activity in developing skeletal muscle correlates with changes in the relative content of the four myogenic regulatory factors. Cardiac α-actin promoter activity, assessed in primary chick myogenic cultures, was activated by endogenous myogenic regulatory factors but was inhibited in the presence of coexpressed MRF4. By exchanging N- and C-terminal domains of MRF4 and MyoD, the N terminus of MRF4 was identified as the mediator of repressive activity, revealing a novel negative regulatory role for MRF4. The relative ratios of myogenic regulatory factors may have fundamental roles in selecting specific muscle genes for activation and/or repression.
AB - Cardiac α-actin is activated early during the development of embryonic skeletal muscle and cardiac myocytes. The gene product remains highly expressed in adult striated cardiac muscle yet is dramatically reduced in skeletal muscle. Activation and repression of cardiac α-actin gene activity in developing skeletal muscle correlates with changes in the relative content of the four myogenic regulatory factors. Cardiac α-actin promoter activity, assessed in primary chick myogenic cultures, was activated by endogenous myogenic regulatory factors but was inhibited in the presence of coexpressed MRF4. By exchanging N- and C-terminal domains of MRF4 and MyoD, the N terminus of MRF4 was identified as the mediator of repressive activity, revealing a novel negative regulatory role for MRF4. The relative ratios of myogenic regulatory factors may have fundamental roles in selecting specific muscle genes for activation and/or repression.
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U2 - 10.1074/jbc.271.49.31688
DO - 10.1074/jbc.271.49.31688
M3 - Article
C2 - 8940190
AN - SCOPUS:0029955957
SN - 0021-9258
VL - 271
SP - 31688
EP - 31694
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 49
ER -