The N-terminal extracellular domain is required for polycystin-1-dependent channel activity

Victor Babich, Wei Zhong Zeng, Byung Il Yeh, Oxana Ibraghimov-Beskrovnaya, Yiqiang Cai, Stefan Somlo, Chou Long Huang

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Autosomal dominant polycystic kidney disease (PKD) is caused by mutation of polycystin-1 or polycystin-2. Polycystin-2 is a Ca2+-permeable cation channel. Polycystin-1 is an integral membrane protein of less defined function. The N-terminal extracellular region of polycystin-1 contains potential motifs for protein and carbohydrate interaction. We now report that expression of polycystin-1 alone in Chinese hamster ovary (CHO) cells and in PKD2-null cells can confer Ca2+-permeable non-selective cation currents. Co-expression of a loss-of-function mutant of polycystin-2 in CHO cells does not reduce polycystin-1-dependent channel activity. A polycystin-1 mutant lacking ∼2900 amino acids of the extracellular region is targeted to the cell surface but does not produce current. Extracellular application of antibodies against the immunoglobulin-like PKD domains reduces polycystin-1-dependent current. These results support the hypothesis that polycystin-1 is a surface membrane receptor that transduces the signal via changes in ionic currents.

Original languageEnglish (US)
Pages (from-to)25582-25589
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number24
DOIs
StatePublished - Jun 11 2004

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Babich, V., Zeng, W. Z., Yeh, B. I., Ibraghimov-Beskrovnaya, O., Cai, Y., Somlo, S., & Huang, C. L. (2004). The N-terminal extracellular domain is required for polycystin-1-dependent channel activity. Journal of Biological Chemistry, 279(24), 25582-25589. https://doi.org/10.1074/jbc.M402829200