The N-terminal region of the DNA-dependent protein kinase catalytic subunit is required for its DNA double-stranded break-mediated activation

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23 Citations (Scopus)

Abstract

Background: The Ku70/80-DNA complex recruits DNA-PKcs to DSBs and results in activation of DNA-PKcs kinase activity. Results: Truncation fragments of DNA-PKcs show that different regions of the protein are required for complete functionality of DNA-PKcs. Conclusion: The N-terminal region of DNA-PKcs is required for its ability to interact with the Ku-DNA complex and its full activation. Significance: We provide insights into the biochemical mechanism required for DNA-PKcs activation.

Original languageEnglish (US)
Pages (from-to)7037-7046
Number of pages10
JournalJournal of Biological Chemistry
Volume288
Issue number10
DOIs
StatePublished - Mar 8 2013

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DNA-Activated Protein Kinase
Double-Stranded DNA Breaks
Catalytic Domain
Chemical activation
DNA
Polynucleotide 5'-Hydroxyl-Kinase
Phosphotransferases

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

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abstract = "Background: The Ku70/80-DNA complex recruits DNA-PKcs to DSBs and results in activation of DNA-PKcs kinase activity. Results: Truncation fragments of DNA-PKcs show that different regions of the protein are required for complete functionality of DNA-PKcs. Conclusion: The N-terminal region of DNA-PKcs is required for its ability to interact with the Ku-DNA complex and its full activation. Significance: We provide insights into the biochemical mechanism required for DNA-PKcs activation.",
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AU - Davis, Anthony J.

AU - Lee, Kyung Jong

AU - Chen, David J.

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N2 - Background: The Ku70/80-DNA complex recruits DNA-PKcs to DSBs and results in activation of DNA-PKcs kinase activity. Results: Truncation fragments of DNA-PKcs show that different regions of the protein are required for complete functionality of DNA-PKcs. Conclusion: The N-terminal region of DNA-PKcs is required for its ability to interact with the Ku-DNA complex and its full activation. Significance: We provide insights into the biochemical mechanism required for DNA-PKcs activation.

AB - Background: The Ku70/80-DNA complex recruits DNA-PKcs to DSBs and results in activation of DNA-PKcs kinase activity. Results: Truncation fragments of DNA-PKcs show that different regions of the protein are required for complete functionality of DNA-PKcs. Conclusion: The N-terminal region of DNA-PKcs is required for its ability to interact with the Ku-DNA complex and its full activation. Significance: We provide insights into the biochemical mechanism required for DNA-PKcs activation.

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