The N-terminal region of the DNA-dependent protein kinase catalytic subunit is required for its DNA double-stranded break-mediated activation

Anthony J. Davis; Kyung Jong Lee; David J. Chen

doi:10.1074/jbc.M112.434498

The N-terminal region of the DNA-dependent protein kinase catalytic subunit is required for its DNA double-stranded break-mediated activation

Anthony J. Davis, Kyung Jong Lee, David J. Chen

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Background: The Ku70/80-DNA complex recruits DNA-PKcs to DSBs and results in activation of DNA-PKcs kinase activity. Results: Truncation fragments of DNA-PKcs show that different regions of the protein are required for complete functionality of DNA-PKcs. Conclusion: The N-terminal region of DNA-PKcs is required for its ability to interact with the Ku-DNA complex and its full activation. Significance: We provide insights into the biochemical mechanism required for DNA-PKcs activation.

Original language	English (US)
Pages (from-to)	7037-7046
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	288
Issue number	10
DOIs	https://doi.org/10.1074/jbc.M112.434498
State	Published - Mar 8 2013

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

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abstract = "Background: The Ku70/80-DNA complex recruits DNA-PKcs to DSBs and results in activation of DNA-PKcs kinase activity. Results: Truncation fragments of DNA-PKcs show that different regions of the protein are required for complete functionality of DNA-PKcs. Conclusion: The N-terminal region of DNA-PKcs is required for its ability to interact with the Ku-DNA complex and its full activation. Significance: We provide insights into the biochemical mechanism required for DNA-PKcs activation.",

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AU - Lee, Kyung Jong

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AB - Background: The Ku70/80-DNA complex recruits DNA-PKcs to DSBs and results in activation of DNA-PKcs kinase activity. Results: Truncation fragments of DNA-PKcs show that different regions of the protein are required for complete functionality of DNA-PKcs. Conclusion: The N-terminal region of DNA-PKcs is required for its ability to interact with the Ku-DNA complex and its full activation. Significance: We provide insights into the biochemical mechanism required for DNA-PKcs activation.

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The N-terminal region of the DNA-dependent protein kinase catalytic subunit is required for its DNA double-stranded break-mediated activation

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