The neuroprotective mechanism of ampicillin in a mouse model of transient forebrain ischemia

Kyung Eon Lee, Kyung Ok Cho, Yun Sik Choi, Seong Yun Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Ampicillin, a β-lactam antibiotic, dose-dependently protects neurons against ischemic brain injury. The present study was performed to investigate the neuroprotective mechanism of ampicillin in a mouse model of transient global forebrain ischemia. Male C57BL/6 mice were anesthetized with halothane and subjected to bilateral common carotid artery occlusion for 40 min. Before transient forebrain ischemia, ampicillin (200 mg/kg, intraperitoneally [i.p.]) or penicillin G (6,000 U/kg or 20,000 U/kg, i.p.) was administered daily for 5 days. The pretreatment with ampicillin but not with penicillin G signif cantly attenuated neuronal damage in the hippocampal CA1 subfield. Mechanistically, the increased activity of matrix metalloproteinases (MMPs) following forebrain ischemia was also attenuated by ampicillin treatment. In addition, the ampicillin treatment reversed increased immunoreactivities to glial fibrillary acidic protein and isolectin B4, markers of astrocytes and microglia, respectively. Furthermore, the ampicillin treatment significantly increased the level of glutamate transporter-1, and dihydrokainic acid (DHK, 10 mg/kg, i.p.), an inhibitor of glutamate transporter-1 (GLT-1), reversed the neuroprotective effect of ampicillin. Taken together, these data indicate that ampicillin provides neuroprotection against ischemia-reperfusion brain injury, possibly through inducing the GLT-1 protein and inhibiting the activity of MMP in the mouse hippocampus.

Original languageEnglish (US)
Pages (from-to)185-192
Number of pages8
JournalKorean Journal of Physiology and Pharmacology
Volume20
Issue number2
DOIs
StatePublished - Mar 1 2016

Fingerprint

Ampicillin
Prosencephalon
Ischemia
Amino Acid Transport System X-AG
Penicillin G
Matrix Metalloproteinases
Brain Injuries
Lactams
Common Carotid Artery
Glial Fibrillary Acidic Protein
Microglia
Neuroprotective Agents
Halothane
Reperfusion Injury
Inbred C57BL Mouse
Lectins
Astrocytes
Hippocampus
Anti-Bacterial Agents
Neurons

Keywords

  • Ampicillin
  • Dihydrokainic acid
  • Glutamate transporter-1
  • Matrix metalloproteinase
  • Transient global forebrain ischemia

ASJC Scopus subject areas

  • Pharmacology
  • Physiology

Cite this

The neuroprotective mechanism of ampicillin in a mouse model of transient forebrain ischemia. / Lee, Kyung Eon; Cho, Kyung Ok; Choi, Yun Sik; Kim, Seong Yun.

In: Korean Journal of Physiology and Pharmacology, Vol. 20, No. 2, 01.03.2016, p. 185-192.

Research output: Contribution to journalArticle

Lee, Kyung Eon ; Cho, Kyung Ok ; Choi, Yun Sik ; Kim, Seong Yun. / The neuroprotective mechanism of ampicillin in a mouse model of transient forebrain ischemia. In: Korean Journal of Physiology and Pharmacology. 2016 ; Vol. 20, No. 2. pp. 185-192.
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