The NLRP3 Inflammasome Mediates In Vivo Innate Immunity to Influenza A Virus through Recognition of Viral RNA

Irving C. Allen, Margaret A. Scull, Chris B. Moore, Eda K. Holl, Erin McElvania-TeKippe, Debra J. Taxman, Elizabeth H. Guthrie, Raymond J. Pickles, Jenny P Y Ting

Research output: Contribution to journalArticle

606 Citations (Scopus)

Abstract

The nucleotide-binding domain and leucine-rich-repeat-containing (NLR) family of pattern-recognition molecules mediate host immunity to various pathogenic stimuli. However, in vivo evidence for the involvement of NLR proteins in viral sensing has not been widely investigated and remains controversial. As a test of the physiologic role of the NLR molecule NLRP3 during RNA viral infection, we explored the in vivo role of NLRP3 inflammasome components during influenza virus infection. Mice lacking Nlrp3, Pycard, or caspase-1, but not Nlrc4, exhibited dramatically increased mortality and a reduced immune response after exposure to the influenza virus. Utilizing analogs of dsRNA (poly(I:C)) and ssRNA (ssRNA40), we demonstrated that an NLRP3-mediated response could be activated by RNA species. Mechanistically, NLRP3 inflammasome activation by the influenza virus was dependent on lysosomal maturation and reactive oxygen species (ROS). Inhibition of ROS induction eliminated IL-1β production in animals during influenza infection. Together, these data place the NLRP3 inflammasome as an essential component in host defense against influenza infection through the sensing of viral RNA.

Original languageEnglish (US)
Pages (from-to)556-565
Number of pages10
JournalImmunity
Volume30
Issue number4
DOIs
StatePublished - Apr 17 2009

Fingerprint

Inflammasomes
Influenza A virus
Viral RNA
Orthomyxoviridae
Innate Immunity
Virus Diseases
Human Influenza
Reactive Oxygen Species
RNA
Caspase 1
Infection
Interleukin-1
Leucine
Immunity
Nucleotides
Mortality

Keywords

  • MOLIMMUNO

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

The NLRP3 Inflammasome Mediates In Vivo Innate Immunity to Influenza A Virus through Recognition of Viral RNA. / Allen, Irving C.; Scull, Margaret A.; Moore, Chris B.; Holl, Eda K.; McElvania-TeKippe, Erin; Taxman, Debra J.; Guthrie, Elizabeth H.; Pickles, Raymond J.; Ting, Jenny P Y.

In: Immunity, Vol. 30, No. 4, 17.04.2009, p. 556-565.

Research output: Contribution to journalArticle

Allen, IC, Scull, MA, Moore, CB, Holl, EK, McElvania-TeKippe, E, Taxman, DJ, Guthrie, EH, Pickles, RJ & Ting, JPY 2009, 'The NLRP3 Inflammasome Mediates In Vivo Innate Immunity to Influenza A Virus through Recognition of Viral RNA', Immunity, vol. 30, no. 4, pp. 556-565. https://doi.org/10.1016/j.immuni.2009.02.005
Allen, Irving C. ; Scull, Margaret A. ; Moore, Chris B. ; Holl, Eda K. ; McElvania-TeKippe, Erin ; Taxman, Debra J. ; Guthrie, Elizabeth H. ; Pickles, Raymond J. ; Ting, Jenny P Y. / The NLRP3 Inflammasome Mediates In Vivo Innate Immunity to Influenza A Virus through Recognition of Viral RNA. In: Immunity. 2009 ; Vol. 30, No. 4. pp. 556-565.
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