The novel differentiation of human blood mononuclear cells into CD1a-negative dendritic cells is stimulated in the absence of exogenous cytokines by an extract prepared from pinecones

W. G. Bradley, R. H. Widen, A. M. Weiser, J. J. Powers, L. B. Fountain, P. Punjwani, S. M. Lofgren, T. Hadzic, R. Klein, W. H. Green, A. Tanaka

Research output: Contribution to journalArticle

9 Scopus citations


The production of dendritic cells, both in-vivo and in-vitro, has become the intense focus of research activities. Common to many of these production protocols is the use of cytokines, typically granulocyte-monocyte colony stimulating factor and either interleukin 4 or tumor necrosis factor alpha or a combination of all three. Herein, we report our findings that a proprietary pinecone extract is capable of in a dose-dependent manner, and in the absence of exogenous cytokines, the rapid differentiation from peripheral blood mononuclear cells of mature CD1a-negative dendritic cells.

Original languageEnglish (US)
Pages (from-to)209-223
Number of pages15
JournalInternational Immunopharmacology
Issue number2
StatePublished - Feb 2003



  • Dendritic cells
  • Peripheral blood mononuclear cells
  • Pinecone extract

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pharmacology

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