The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene

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Abstract

This paper presents the nucleotide sequence of the Herpes Simplex Virus thymidine kinase (tk) gene. The positions on the DNA sequence corresponding to the 5′ and 3′ termini of tk messenger RNA have been mapped. The mRNA termini are separated by slightly more than 1,300 nucleotides. The same 2,300 nucleotide segment of tk coding strand DNA is fully protected from S1 nuclease digestion when hybridized to tk mRNA. The location and size of the mRNA-coding segment corresponds to a region of the viral DNA that is essential for tk gene expression in microinjected frog oocytes. The nucleotide sequence of the HSV tk gene exhibits an open translational reading frame of 376 codons that extends from the methionine codon most proximal to the 5′ terminus of tk mRNA to a USA stop codon ∼70 nucleotides from the poly-A addition site. The results of these experiments indicate that the tk gene is not interrupted by intervening DNA sequences, and that certain oligonucleotide sequences adjacent to the termini of the tk gene are homologous to similarly positioned sequences common to structural genes of eukaryotic cells.

Original languageEnglish (US)
Pages (from-to)5949-5964
Number of pages16
JournalNucleic Acids Research
Volume8
Issue number24
DOIs
StatePublished - Dec 20 1980

Fingerprint

Thymidine Kinase
Simplexvirus
Nucleotides
Viruses
Messenger RNA
Virus
Genes
Gene
DNA sequences
DNA Sequence
DNA
Coding
Oocyte
Oligonucleotides
Codon
Gene expression
Gene Expression
Adjacent
Nucleotide sequence
Poly A

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene. / Mcknight, Steven L.

In: Nucleic Acids Research, Vol. 8, No. 24, 20.12.1980, p. 5949-5964.

Research output: Contribution to journalArticle

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