The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly

Arturo V. Orjalo, Alexei Arnaoutov, Zhouxin Shen, Yekaterina Boyarchuk, Samantha G. Zeitlin, Beatriz Fontoura, Steven Briggs, Mary Dasso, Douglass J. Forbes

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

The Nup107-160 complex is a critical subunit of the nuclear pore. This complex localizes to kinetochores in mitotic mammalian cells, where its function is unknown. To examine Nup107-160 complex recruitment to kinetochores, we stained human cells with antisera to four complex components. Each antibody stained not only kinetochores but also prometaphase spindle poles and proximal spindle fibers, mirroring the dual prometaphase localization of the spindle checkpoint proteins Mad1, Mad2, Bub3, and Cdc20. Indeed, expanded crescents of the Nup107-160 complex encircled unattached kinetochores, similar to the hyperaccumulation observed of dynamic outer kinetochore checkpoint proteins and motors at unattached kinetochores. In mitotic Xenopus egg extracts, the Nup107-160 complex localized throughout reconstituted spindles. When the Nup107-160 complex was depleted from extracts, the spindle checkpoint remained intact, but spindle assembly was rendered strikingly defective. Microtubule nucleation around sperm centrosomes seemed normal, but the microtubules quickly disassembled, leaving largely unattached sperm chromatin. Notably, Ran-GTP caused normal assembly of microtubule asters in depleted extracts, indicating that this defect was upstream of Ran or independent of it. We conclude that the Nup107-160 complex is dynamic in mitosis and that it promotes spindle assembly in a manner that is distinct from its functions at interphase nuclear pores.

Original languageEnglish (US)
Pages (from-to)3806-3818
Number of pages13
JournalMolecular Biology of the Cell
Volume17
Issue number9
DOIs
StatePublished - Sep 2006

Fingerprint

Nuclear Pore Complex Proteins
Kinetochores
Microtubules
Prometaphase
Nuclear Pore
Mad2 Proteins
Spermatozoa
Spindle Poles
Centrosome
Interphase
Xenopus
Guanosine Triphosphate
Mitosis
Chromatin
Ovum
Immune Sera
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Orjalo, A. V., Arnaoutov, A., Shen, Z., Boyarchuk, Y., Zeitlin, S. G., Fontoura, B., ... Forbes, D. J. (2006). The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. Molecular Biology of the Cell, 17(9), 3806-3818. https://doi.org/10.1091/mbc.E05-11-1061

The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. / Orjalo, Arturo V.; Arnaoutov, Alexei; Shen, Zhouxin; Boyarchuk, Yekaterina; Zeitlin, Samantha G.; Fontoura, Beatriz; Briggs, Steven; Dasso, Mary; Forbes, Douglass J.

In: Molecular Biology of the Cell, Vol. 17, No. 9, 09.2006, p. 3806-3818.

Research output: Contribution to journalArticle

Orjalo, AV, Arnaoutov, A, Shen, Z, Boyarchuk, Y, Zeitlin, SG, Fontoura, B, Briggs, S, Dasso, M & Forbes, DJ 2006, 'The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly', Molecular Biology of the Cell, vol. 17, no. 9, pp. 3806-3818. https://doi.org/10.1091/mbc.E05-11-1061
Orjalo, Arturo V. ; Arnaoutov, Alexei ; Shen, Zhouxin ; Boyarchuk, Yekaterina ; Zeitlin, Samantha G. ; Fontoura, Beatriz ; Briggs, Steven ; Dasso, Mary ; Forbes, Douglass J. / The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly. In: Molecular Biology of the Cell. 2006 ; Vol. 17, No. 9. pp. 3806-3818.
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