The oncogenic RNA-binding protein Musashi1 is regulated by tumor suppressor miRNAs

Dat T. Vo, Mei Qiao, Andrew D. Smith, Suzanne C. Burns, Andrew J. Brenner, Luiz O.F. Penalva

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Musashi1 (Msi1) is an evolutionarily conserved RNA-binding protein that has been implicated in processes like stem cell fate, nervous system development and tumorigenesis via its activities as a specific regulator of translation. While Msi1 is barely detected in normal adult tissue, it has been observed to be highly expressed in numerous tumor types (e.g., breast, colon, medulloblastoma, glioblastoma and et cetera). Unfortunately, the molecular cues that are responsible for Msi1 upregulation in cancer cells are largely unknown. Tumor suppressor microRNAs (miRNAs) are known for targeting genes with oncogenic properties like Msi1 and for being either downregulated or deleted in tumor tissue. We observed that Msi1 long 3′UTR region is potentially targeted by several tumor suppressor miRNAs (miR-34a, -101, -128, -137 and -138). Western blotting of endogenous Msi1 protein as well as luciferase assays confirmed Msi1 regulation by these tumor suppressor miRNAs. Furthermore, we observed when examining different cellular states that these miRNAs and Msi1 have opposite expression profiles. Cell proliferation inhibition induced by the tumor suppressor miRNAs was partially rescued by Msi1 transgenic expression. We conclude that tumor suppressor miRNAs are direct and influential regulators of Msi1, affecting its expression pattern during tumorigenesis of malignant nervous system tumors.

Original languageEnglish (US)
JournalRNA Biology
Volume8
Issue number5
DOIs
StatePublished - Jan 1 2011
Externally publishedYes

Fingerprint

RNA-Binding Proteins
MicroRNAs
Neoplasms
Carcinogenesis
Nervous System Neoplasms
Medulloblastoma
Gene Targeting
Glioblastoma
Luciferases
Nervous System
Cues
Colon
Up-Regulation
Stem Cells
Down-Regulation
Western Blotting
Cell Proliferation
Breast Neoplasms

Keywords

  • Cancer
  • Cancer stem cell
  • MicroRNA
  • Musashi1
  • Post-transcriptional gene regulation
  • RNA-binding protein
  • Tumor suppressor
  • Tumorigenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Vo, D. T., Qiao, M., Smith, A. D., Burns, S. C., Brenner, A. J., & Penalva, L. O. F. (2011). The oncogenic RNA-binding protein Musashi1 is regulated by tumor suppressor miRNAs. RNA Biology, 8(5). https://doi.org/10.4161/rna.8.5.16041

The oncogenic RNA-binding protein Musashi1 is regulated by tumor suppressor miRNAs. / Vo, Dat T.; Qiao, Mei; Smith, Andrew D.; Burns, Suzanne C.; Brenner, Andrew J.; Penalva, Luiz O.F.

In: RNA Biology, Vol. 8, No. 5, 01.01.2011.

Research output: Contribution to journalArticle

Vo, Dat T. ; Qiao, Mei ; Smith, Andrew D. ; Burns, Suzanne C. ; Brenner, Andrew J. ; Penalva, Luiz O.F. / The oncogenic RNA-binding protein Musashi1 is regulated by tumor suppressor miRNAs. In: RNA Biology. 2011 ; Vol. 8, No. 5.
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