The oocyte-to-embryo transition

Research output: Chapter in Book/Report/Conference proceedingChapter

25 Citations (Scopus)

Abstract

The oocyte-to-embryo transition refers to the process whereby a fully grown, relatively quiescent oocyte undergoes maturation, fertilization, and is converted into a developmentally active, mitotically dividing embryo, arguably one of the most dramatic transitions in biology. This transition occurs very rapidly in Caenorhabditis elegans, with fertilization of a new oocyte occurring every 23 min and the first mitotic division occurring 45 min later. Molecular events regulating this transition must be very precisely timed. This chapter reviews our current understanding of the coordinated temporal regulation of different events during this transition. We divide the oocyte-to-embryo transition into a number of component processes, which are coordinated primarily through the MBK-2 kinase, whose activation is intimately tied to completion of meiosis, and the OMA-1/OMA-2 proteins, whose expression and functions span multiple processes during this transition. The oocyte-to-embryo transition occurs in the absence of de novo transcription, and all the factors required for the process, whether mRNA or protein, are already present within the oocyte. Therefore, all regulation of this transition is posttranscriptional. The combination of asymmetric partitioning of maternal factors, protein modification-mediated functional switching, protein degradation, and highly regulated translational repression ensure a smooth oocyte-to-embryo transition. We will highlight protein degradation and translational repression, two posttranscriptional processes which play particularly critical roles in this transition.

Original languageEnglish (US)
Title of host publicationAdvances in Experimental Medicine and Biology
Pages351-372
Number of pages22
Volume757
DOIs
StatePublished - 2013

Publication series

NameAdvances in Experimental Medicine and Biology
Volume757
ISSN (Print)00652598

Fingerprint

Oocytes
Embryonic Structures
Proteins
Fertilization
Proteolysis
Degradation
Transcription
Phosphotransferases
Caenorhabditis elegans
Meiosis
Chemical activation
Messenger RNA
Transcription Factors
Mothers

Keywords

  • 3′UTR
  • Asymmetric partitioning
  • MBK-2
  • OMA-1
  • Oocyte maturation
  • Oocyte-to-embryo transition
  • Protein degradation
  • RNA binding
  • Translational repression
  • ZIF-1

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Robertson, S., & Lin, R. (2013). The oocyte-to-embryo transition. In Advances in Experimental Medicine and Biology (Vol. 757, pp. 351-372). (Advances in Experimental Medicine and Biology; Vol. 757). https://doi.org/10.1007/978-1-4614-4015-4-12

The oocyte-to-embryo transition. / Robertson, Scott; Lin, Rueyling.

Advances in Experimental Medicine and Biology. Vol. 757 2013. p. 351-372 (Advances in Experimental Medicine and Biology; Vol. 757).

Research output: Chapter in Book/Report/Conference proceedingChapter

Robertson, S & Lin, R 2013, The oocyte-to-embryo transition. in Advances in Experimental Medicine and Biology. vol. 757, Advances in Experimental Medicine and Biology, vol. 757, pp. 351-372. https://doi.org/10.1007/978-1-4614-4015-4-12
Robertson S, Lin R. The oocyte-to-embryo transition. In Advances in Experimental Medicine and Biology. Vol. 757. 2013. p. 351-372. (Advances in Experimental Medicine and Biology). https://doi.org/10.1007/978-1-4614-4015-4-12
Robertson, Scott ; Lin, Rueyling. / The oocyte-to-embryo transition. Advances in Experimental Medicine and Biology. Vol. 757 2013. pp. 351-372 (Advances in Experimental Medicine and Biology).
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