The optional E.coli prr locus encodes a latent form of phage T4-induced anticodon nuclease

Ruth Levitz, Daphne Chapman, Michal Amitsur, Robert Green, Larry Snyder, Gabriel Kaufmann

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The optional Escherichia coli prr locus restricts phage T4 mutants lacking polynucleotide kinase or RNA ligase. Underlying this restriction is the specific manifestation of the T4-induced anticodon nuclease, an enzyme which triggers the cleavage-ligation of the host tRNALys. We report here the molecular cloning, nucleotide sequence and mutational analysis of prr-associated DNA. The results indicate that prr encodes a latent form of anticodon nuclease consisting of a core enzyme and cognate masking agents. They suggest that the T4-encoded factors of anticodon nuclease counteract the prr-encoded masking agents, thus activating the latent enzyme. The encoding of a tRNA cleavage-ligation pathway by two separate genetic systems which cohabitate E.coli may provide a clue to the evolution of RNA splicing mechanisms mediated by proteins.

Original languageEnglish (US)
Pages (from-to)1383-1389
Number of pages7
JournalEMBO Journal
Volume9
Issue number5
StatePublished - 1990

Fingerprint

Bacteriophage T4
Bacteriophages
Escherichia coli
Ligation
Enzymes
RNA, Transfer, Lys
Polynucleotide 5'-Hydroxyl-Kinase
RNA
RNA Splicing
Cloning
Molecular Cloning
Ligases
Transfer RNA
Sequence Analysis
Nucleotides
DNA
anticodon nuclease
Proteins

Keywords

  • Bacterial restriction system
  • Polynucleotide kinase
  • RNA ligase
  • T4 stp gene
  • TRNA splicing

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Levitz, R., Chapman, D., Amitsur, M., Green, R., Snyder, L., & Kaufmann, G. (1990). The optional E.coli prr locus encodes a latent form of phage T4-induced anticodon nuclease. EMBO Journal, 9(5), 1383-1389.

The optional E.coli prr locus encodes a latent form of phage T4-induced anticodon nuclease. / Levitz, Ruth; Chapman, Daphne; Amitsur, Michal; Green, Robert; Snyder, Larry; Kaufmann, Gabriel.

In: EMBO Journal, Vol. 9, No. 5, 1990, p. 1383-1389.

Research output: Contribution to journalArticle

Levitz, R, Chapman, D, Amitsur, M, Green, R, Snyder, L & Kaufmann, G 1990, 'The optional E.coli prr locus encodes a latent form of phage T4-induced anticodon nuclease', EMBO Journal, vol. 9, no. 5, pp. 1383-1389.
Levitz R, Chapman D, Amitsur M, Green R, Snyder L, Kaufmann G. The optional E.coli prr locus encodes a latent form of phage T4-induced anticodon nuclease. EMBO Journal. 1990;9(5):1383-1389.
Levitz, Ruth ; Chapman, Daphne ; Amitsur, Michal ; Green, Robert ; Snyder, Larry ; Kaufmann, Gabriel. / The optional E.coli prr locus encodes a latent form of phage T4-induced anticodon nuclease. In: EMBO Journal. 1990 ; Vol. 9, No. 5. pp. 1383-1389.
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