The paired-like homeo box gene MHox is required for early events of skeletogenesis in multiple lineages

J. F. Martin, A. Bradley, E. N. Olson

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Formation of cartilage and bone involves sequential processes in which undifferentiated mesenchyme aggregates into primordial condensations that subsequently grow and differentiate, eventually forming the adult skeleton. Although much has been learned about the structural molecules that compose cartilage and bone, little is known about the nuclear factors that regulate chondrogenesis and osteogenesis. MHox is a homeo box-containing gene that is expressed in the mesenchyme of facial, limb, and vertebral skeletal precursors during mouse embryogenesis. MHox expression has been shown to require epithelial-derived signals, suggesting that MHox may regulate the epithelial-mesenchymal interactions required for skeletal organogenesis. To determine the functions of MHox, we generated a loss-of-function mutation in the MHox gene. Mice homozygous for a mutant MHox allele die soon after birth and exhibit defects of skeletogenesis, involving the loss or malformation of craniofacial, limb, and vertebral skeletal structures. The affected skeletal elements are derived from the cranial neural crest, as well as somitic and lateral mesoderm. Analysis of the mutant phenotype during ontogeny demonstrated a defect in the formation and growth of chondrogenic and osteogenic precursors. These findings provide evidence that MHox regulates the formation of preskeletal condensations from undifferentiated mesenchyme.

Original languageEnglish (US)
Pages (from-to)1237-1249
Number of pages13
JournalGenes and Development
Volume9
Issue number10
StatePublished - 1995

Fingerprint

Homeobox Genes
Mesoderm
Osteogenesis
Cartilage
Extremities
Chondrogenesis
Organogenesis
Neural Crest
Skeleton
Embryonic Development
Alleles
Phenotype
Bone and Bones
Mutation
Growth
Genes

Keywords

  • epithelial-mesenchymal interactions
  • homeo box
  • MHox gene
  • mouse embryogenesis
  • skeletal organogenesis

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

The paired-like homeo box gene MHox is required for early events of skeletogenesis in multiple lineages. / Martin, J. F.; Bradley, A.; Olson, E. N.

In: Genes and Development, Vol. 9, No. 10, 1995, p. 1237-1249.

Research output: Contribution to journalArticle

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