The polyamine spermine protects against high salt stress in Arabidopsis thaliana

Koji Yamaguchi, Yoshihiro Takahashi, Thomas Berberich, Akihiko Imai, Atsushi Miyazaki, Taku Takahashi, Anthony Michael, Tomonobu Kusano

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

It is well known that changes in abiotic conditions such as the concentration of ions, temperature and humidity lead to modulation of polyamine contents in plants. However, little is known about the relevant parts these polyamines play in abiotic stress responses. Here we addressed a specific role of spermine during high salt stress using an Arabidopsis double knockout-mutant plant (acl5/spms) which cannot produce spermine. The mutant showed higher sensitivity to high salt than wild type plants. This phenotype was cured by exogenous spermine but not by the other polyamines putrescine and spermidine, suggesting a strong link between spermine-deficiency and NaCl-hypersensitivity. The mutant was also hypersensitive to high levels of KCl but not to MgCl2 or to high osmoticum. NaCl-hypersensitivity of the mutant was compromised by treatment with Ca2+ channel blockers. Moreover, the mutant showed poor growth on Ca2+-depleted Murashige-Skoog agar media. The data suggest that the absence of spermine causes an imbalance in Ca2+ homeostasis in the mutant plant. Based on the data obtained, we propose a model for a role of spermine in high salt stress responses.

Original languageEnglish (US)
Pages (from-to)6783-6788
Number of pages6
JournalFEBS Letters
Volume580
Issue number30
DOIs
StatePublished - Dec 22 2006

Fingerprint

Spermine
Polyamines
Arabidopsis
Salts
Hypersensitivity
Magnesium Chloride
Putrescine
Spermidine
Humidity
Agar
Atmospheric humidity
Homeostasis
Modulation
Ions
Phenotype
Temperature
Growth

Keywords

  • Arabidopsis thaliana
  • Calcium homeostasis
  • Polyamine
  • Salt stress
  • Spermine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Yamaguchi, K., Takahashi, Y., Berberich, T., Imai, A., Miyazaki, A., Takahashi, T., ... Kusano, T. (2006). The polyamine spermine protects against high salt stress in Arabidopsis thaliana. FEBS Letters, 580(30), 6783-6788. https://doi.org/10.1016/j.febslet.2006.10.078

The polyamine spermine protects against high salt stress in Arabidopsis thaliana. / Yamaguchi, Koji; Takahashi, Yoshihiro; Berberich, Thomas; Imai, Akihiko; Miyazaki, Atsushi; Takahashi, Taku; Michael, Anthony; Kusano, Tomonobu.

In: FEBS Letters, Vol. 580, No. 30, 22.12.2006, p. 6783-6788.

Research output: Contribution to journalArticle

Yamaguchi, K, Takahashi, Y, Berberich, T, Imai, A, Miyazaki, A, Takahashi, T, Michael, A & Kusano, T 2006, 'The polyamine spermine protects against high salt stress in Arabidopsis thaliana', FEBS Letters, vol. 580, no. 30, pp. 6783-6788. https://doi.org/10.1016/j.febslet.2006.10.078
Yamaguchi K, Takahashi Y, Berberich T, Imai A, Miyazaki A, Takahashi T et al. The polyamine spermine protects against high salt stress in Arabidopsis thaliana. FEBS Letters. 2006 Dec 22;580(30):6783-6788. https://doi.org/10.1016/j.febslet.2006.10.078
Yamaguchi, Koji ; Takahashi, Yoshihiro ; Berberich, Thomas ; Imai, Akihiko ; Miyazaki, Atsushi ; Takahashi, Taku ; Michael, Anthony ; Kusano, Tomonobu. / The polyamine spermine protects against high salt stress in Arabidopsis thaliana. In: FEBS Letters. 2006 ; Vol. 580, No. 30. pp. 6783-6788.
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