The power of real-time PCR

Mark A. Valasek, Joyce J. Repa

Research output: Contribution to journalArticle

341 Scopus citations

Abstract

In recent years, real-time polymerase chain reaction (PCR) has emerged as a robust and widely used methodology for biological investigation because it can detect and quantify very small amounts of specific nucleic acid sequences. As a research tool, a major application of this technology is the rapid and accurate assessment of changes in gene expression as a result of physiology, pathophysiology, or development. This method can be applied to model systems to measure responses to experimental stimuli and to gain insight into potential changes in protein level and function. Thus physiology can be correlated with molecular events to gain a better understanding of biological processes. For clinical molecular diagnostics, real-time PCR can be used to measure viral or bacterial loads or evaluate cancer status. Here, we discuss the basic concepts, chemistries, and instrumentation of real-time PCR and include present applications and future perspectives for this technology in biomedical sciences and in life science education.

Original languageEnglish (US)
Pages (from-to)151-159
Number of pages9
JournalAmerican Journal of Physiology - Advances in Physiology Education
Volume29
Issue number3
DOIs
StatePublished - Sep 2005

Keywords

  • Adiponectin receptor
  • Method
  • Quantitative real-time polymerase chain reaction
  • Review

ASJC Scopus subject areas

  • Physiology

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