The presence of platelet-activating factor binding sites in human myometrium and their role in uterine contraction

Y. Zhu, R. A. Word, J. M. Johnston

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

It has recently been suggested that platelet-activating factor is involved in a number of processes associated with reproductive biology. We have demonstrated a critical role for this autacoid in the initiation and maintenance of parturition both in animals and man. In the present investigation, it has been demonstrated that platelet-activating factor affects contraction in strips of human myometrium at concentrations as low as 10−10 mol/L. A platelet-activating factor receptor has been identified and characterized in human myometrium. Platelet-activating factor acts to cause an increase in intracellular Ca2+ concentration of isolated myometrial smooth muscle cells in culture and an increase in the phosphorylation of the 20 kd light chain of myosin in a concentration-dependent fashion. The presence of platelet-activating factor receptors in human myometrium, the observed increase in Ca+2 concentration and phosphorylation of myosin light chain in the presence of platelet-activating factor provide further support for the importance of this autacoid in the initation of parturition.

Original languageEnglish (US)
Pages (from-to)1222-1227
Number of pages6
JournalAmerican journal of obstetrics and gynecology
Volume166
Issue number4
DOIs
StatePublished - Jan 1 1992

    Fingerprint

Keywords

  • Platelet-activating factor
  • calcium
  • myometrium
  • myosin light chain phosphorylation
  • platelet-activating factor receptors

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this