The production of mass populations of anucleate cytoplasms

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Until recently anucleate cytoplasms could only be obtained by microsurgery. The discovery of Carter (1967) that under certain circumstances cytochalasin B (CB) from the mold Helminthosporium dematiodium could cause the loss of the nucleus from the cell, has now been applied by the author to obtain anucleate cytoplasms in larger quantities. The technique which makes use of CB and centrifugation of cell suspensions is clearly described. The efficiency of the method depends largely on the CB concentration, time and force of the centrifugation and temperature, but any or all of these parameters can be manipulated to meet the requirements of a particular experiment. (Woerdeman - Amsterdam)

Original languageEnglish (US)
Title of host publicationMETH.CELL BIOL.
Pages203-210
Number of pages8
VolumeNo.7
StatePublished - 1973

Fingerprint

Cytochalasin B
Cytoplasm
Centrifugation
Helminthosporium
Population
Microsurgery
Cell Nucleus
Suspensions
Fungi
Temperature

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Wright, W. E. (1973). The production of mass populations of anucleate cytoplasms. In METH.CELL BIOL. (Vol. No.7, pp. 203-210)

The production of mass populations of anucleate cytoplasms. / Wright, W. E.

METH.CELL BIOL.. Vol. No.7 1973. p. 203-210.

Research output: Chapter in Book/Report/Conference proceedingChapter

Wright, WE 1973, The production of mass populations of anucleate cytoplasms. in METH.CELL BIOL.. vol. No.7, pp. 203-210.
Wright WE. The production of mass populations of anucleate cytoplasms. In METH.CELL BIOL.. Vol. No.7. 1973. p. 203-210
Wright, W. E. / The production of mass populations of anucleate cytoplasms. METH.CELL BIOL.. Vol. No.7 1973. pp. 203-210
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