TY - JOUR
T1 - The production of soluble interleukin 4 receptors is preferentially regulated by the murine Th2 cell subset
AU - Fernandez-Botran, Rafael
AU - Chilton, Paula M.
AU - Ma, Yuhe
AU - Windsor, Jana L.
AU - Street, Nancy E.
N1 - Funding Information:
We are indebted to Dr Michael Widmer and Dr Tony Troutt "Immunex R + D Corporation# for their generous supply of sIL!3r and M0 and M1 antibodies and to Dr Peter Linsley "Bristol!Myers Squibb# for the human CTLA!3Ig[ We also thank Dr Thomas A[ Wynn "Laboratory of Parasitic Diseases\ NIAID\ Bethesda\ MD# for his help in the design of PCR primers^ and Dr Michael T[ Berton "Department of Microbiology\ The University of Texas Health Sciences Center at San Antonio\ TX# for providing CD39L!transfected cells[ This work was supported in part by American Heart Association grant No[ 82900039 and NIH grant AI!23516 "to R[F[B[# and NIH grant No[ CA!44155 "to N[E[S[#[
PY - 1997/3
Y1 - 1997/3
N2 - In order to understand how the endogenous production of soluble IL-4 receptors (sIL-4r) is regulated, the authors tested prototypic clones of Th1 and Th2 murine CD4+ T cell subsets for their ability to regulate their expression of sIL-4r. Results showed that although both types of clones produced low levels of sIL-4r under resting conditions, only the Th2 clones upregulated sIL-4r expression following antigenic stimulation. Inhibition of endogenous IL-4 with a neutralizing anti-IL-4 mAb had only a minor (~ 20%) inhibitory effect on sIL-4r production by the Th2 cells, and addition of rIL-4 to Th1 cells resulted only in a modest two-fold increase in sIL-4r levels, suggesting that IL-4 is not the only factor that regulates sIL-4r production and that the ability of Th2 clones to upregulate sIL-4r expression can be relatively independent of IL-4. Indeed, the production of sIL-4r by Th2 cells was found to be regulated by cell contact and/or IL-1-mediated signals. Transcripts for both sIL-4r and mIL-4r were detected by RT-PCR on both resting and activated Th1 and Th2 cells, with the relative levels of expression being moderately higher in the Th2 clones. Moreover, the expression of sIL-4r-specific transcripts appeared to increase to a greater extent than those of mIL-4r after activation of Th2 cells with APCs, both in the presence and absence of antigen. Taken together, these results predict that increased sIL-4r production in vivo might be preferentially associated with Th2-type responses and indicate that even though the production of IL-4 and sIL-4r is mediated by the same cells (i.e. Th2 cells), the synthesis of sIL-4r can be regulated independently from that of IL-4 through alternative signals such as cell contact and/or IL-1. These properties may allow for changing ratios of sIL-4r to IL-4 and sIL-4r to mIL-4r during different phases of an immune response and are consistent with a regulatory role for sIL-4r on IL-4 activity in vivo.
AB - In order to understand how the endogenous production of soluble IL-4 receptors (sIL-4r) is regulated, the authors tested prototypic clones of Th1 and Th2 murine CD4+ T cell subsets for their ability to regulate their expression of sIL-4r. Results showed that although both types of clones produced low levels of sIL-4r under resting conditions, only the Th2 clones upregulated sIL-4r expression following antigenic stimulation. Inhibition of endogenous IL-4 with a neutralizing anti-IL-4 mAb had only a minor (~ 20%) inhibitory effect on sIL-4r production by the Th2 cells, and addition of rIL-4 to Th1 cells resulted only in a modest two-fold increase in sIL-4r levels, suggesting that IL-4 is not the only factor that regulates sIL-4r production and that the ability of Th2 clones to upregulate sIL-4r expression can be relatively independent of IL-4. Indeed, the production of sIL-4r by Th2 cells was found to be regulated by cell contact and/or IL-1-mediated signals. Transcripts for both sIL-4r and mIL-4r were detected by RT-PCR on both resting and activated Th1 and Th2 cells, with the relative levels of expression being moderately higher in the Th2 clones. Moreover, the expression of sIL-4r-specific transcripts appeared to increase to a greater extent than those of mIL-4r after activation of Th2 cells with APCs, both in the presence and absence of antigen. Taken together, these results predict that increased sIL-4r production in vivo might be preferentially associated with Th2-type responses and indicate that even though the production of IL-4 and sIL-4r is mediated by the same cells (i.e. Th2 cells), the synthesis of sIL-4r can be regulated independently from that of IL-4 through alternative signals such as cell contact and/or IL-1. These properties may allow for changing ratios of sIL-4r to IL-4 and sIL-4r to mIL-4r during different phases of an immune response and are consistent with a regulatory role for sIL-4r on IL-4 activity in vivo.
KW - CD4 T cell subsets
KW - IL-4
KW - Immunoregulation
KW - Soluble interleukin 4 receptor
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U2 - 10.1006/cyto.1996.0151
DO - 10.1006/cyto.1996.0151
M3 - Article
C2 - 9126705
AN - SCOPUS:0031104947
SN - 1043-4666
VL - 9
SP - 166
EP - 177
JO - Cytokine
JF - Cytokine
IS - 3
ER -