The proline/arginine-rich domain is a major determinant of dynamin self-activation

Barbara Barylko, Lei Wang, Derk D. Binns, Justin A. Ross, Tara C. Tassin, Katie A. Collins, David M. Jameson, Joseph P. Albanesi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Dynamins induce membrane vesiculation during endocytosis and Golgi budding in a process that requires assembly-dependent GTPase activation. Brain-specific dynamin 1 has a weaker propensity to self-assemble and self-activate than ubiquitously expressed dynamin 2. Here we show that dynamin 3, which has important functions in neuronal synapses, shares the self-assembly and GTPase activation characteristics of dynamin 2. Analysis of dynamin hybrids and of dynamin 1-dynamin 2 and dynamin 1-dynamin 3 heteropolymers reveals that concentration-dependent GTPase activation is suppressed by the C-terminal proline/arginine-rich domain of dynamin 1. Dynamin proline/arginine-rich domains also mediate interactions with SH3 domain-containing proteins and thus regulate both self-association and heteroassociation of dynamins.

Original languageEnglish (US)
Pages (from-to)10592-10594
Number of pages3
JournalBiochemistry
Volume49
Issue number50
DOIs
StatePublished - Dec 21 2010

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Dynamin I
Dynamins
Dynamin II
Proline
Dynamin III
Arginine
GTP Phosphohydrolases
Chemical activation
src Homology Domains
Endocytosis
Synapses
Self assembly
Brain
Association reactions
Membranes
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

The proline/arginine-rich domain is a major determinant of dynamin self-activation. / Barylko, Barbara; Wang, Lei; Binns, Derk D.; Ross, Justin A.; Tassin, Tara C.; Collins, Katie A.; Jameson, David M.; Albanesi, Joseph P.

In: Biochemistry, Vol. 49, No. 50, 21.12.2010, p. 10592-10594.

Research output: Contribution to journalArticle

Barylko, B, Wang, L, Binns, DD, Ross, JA, Tassin, TC, Collins, KA, Jameson, DM & Albanesi, JP 2010, 'The proline/arginine-rich domain is a major determinant of dynamin self-activation', Biochemistry, vol. 49, no. 50, pp. 10592-10594. https://doi.org/10.1021/bi101343p
Barylko B, Wang L, Binns DD, Ross JA, Tassin TC, Collins KA et al. The proline/arginine-rich domain is a major determinant of dynamin self-activation. Biochemistry. 2010 Dec 21;49(50):10592-10594. https://doi.org/10.1021/bi101343p
Barylko, Barbara ; Wang, Lei ; Binns, Derk D. ; Ross, Justin A. ; Tassin, Tara C. ; Collins, Katie A. ; Jameson, David M. ; Albanesi, Joseph P. / The proline/arginine-rich domain is a major determinant of dynamin self-activation. In: Biochemistry. 2010 ; Vol. 49, No. 50. pp. 10592-10594.
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