The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators

Daeyoup Lee; Elena Ezhkova; Bing Li; Samantha G. Pattenden; William P. Tansey; Jerry L. Workman

doi:10.1016/j.cell.2005.08.015

The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators

Daeyoup Lee, Elena Ezhkova, Bing Li, Samantha G. Pattenden, William P. Tansey, Jerry L. Workman

Research output: Contribution to journal › Article › peer-review

155 Scopus citations

Abstract

Promoter recruitment of the Saccharomyces cerevisiae SAGA histone acetyltransferase complex is required for RNA polymerase II-dependent transcription of several genes. SAGA is targeted to promoters through interactions with sequence-specific DNA binding transcriptional activators and facilitates preinitiation-complex assembly and transcription. Here, we show that the 19S proteasome regulatory particle (19S RP) alters SAGA to stimulate its interaction with transcriptional activators. The ATPase components of the 19S RP are required for stimulation of SAGA/activator interactions and enhance SAGA recruitment to promoters. Proteasomal ATPases genetically interact with SAGA, and their inhibition reduces global histone H3 acetylation levels and SAGA recruitment to target promoters in vivo. These results indicate that the 19S RP modulates SAGA complex using its ATPase components, thereby facilitating subsequent transcription events at promoters.

Original language	English (US)
Pages (from-to)	423-436
Number of pages	14
Journal	Cell
Volume	123
Issue number	3
DOIs	https://doi.org/10.1016/j.cell.2005.08.015
State	Published - Nov 4 2005

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2005.08.015

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title = "The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators",

abstract = "Promoter recruitment of the Saccharomyces cerevisiae SAGA histone acetyltransferase complex is required for RNA polymerase II-dependent transcription of several genes. SAGA is targeted to promoters through interactions with sequence-specific DNA binding transcriptional activators and facilitates preinitiation-complex assembly and transcription. Here, we show that the 19S proteasome regulatory particle (19S RP) alters SAGA to stimulate its interaction with transcriptional activators. The ATPase components of the 19S RP are required for stimulation of SAGA/activator interactions and enhance SAGA recruitment to promoters. Proteasomal ATPases genetically interact with SAGA, and their inhibition reduces global histone H3 acetylation levels and SAGA recruitment to target promoters in vivo. These results indicate that the 19S RP modulates SAGA complex using its ATPase components, thereby facilitating subsequent transcription events at promoters.",

author = "Daeyoup Lee and Elena Ezhkova and Bing Li and Pattenden, {Samantha G.} and Tansey, {William P.} and Workman, {Jerry L.}",

note = "Funding Information: We thank F. Winston, S.A. Johnston, J. Reece, S. Tan, and K. K. Lee for yeast strains, valuable antibodies, recombinant proteins, and purification of SLIK. We want to acknowledge Jos{\'e} Guti{\'e}rrez for suggesting experimental schemes. We also wish to thank Mark Chandy for critical reading of the manuscript. This work was supported by NIGMS grants GM047867 to J.L.W. and GM067728 to W.P.T. ",

year = "2005",

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doi = "10.1016/j.cell.2005.08.015",

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T1 - The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators

AU - Lee, Daeyoup

AU - Ezhkova, Elena

AU - Li, Bing

AU - Pattenden, Samantha G.

AU - Tansey, William P.

AU - Workman, Jerry L.

N1 - Funding Information: We thank F. Winston, S.A. Johnston, J. Reece, S. Tan, and K. K. Lee for yeast strains, valuable antibodies, recombinant proteins, and purification of SLIK. We want to acknowledge José Gutiérrez for suggesting experimental schemes. We also wish to thank Mark Chandy for critical reading of the manuscript. This work was supported by NIGMS grants GM047867 to J.L.W. and GM067728 to W.P.T.

PY - 2005/11/4

Y1 - 2005/11/4

N2 - Promoter recruitment of the Saccharomyces cerevisiae SAGA histone acetyltransferase complex is required for RNA polymerase II-dependent transcription of several genes. SAGA is targeted to promoters through interactions with sequence-specific DNA binding transcriptional activators and facilitates preinitiation-complex assembly and transcription. Here, we show that the 19S proteasome regulatory particle (19S RP) alters SAGA to stimulate its interaction with transcriptional activators. The ATPase components of the 19S RP are required for stimulation of SAGA/activator interactions and enhance SAGA recruitment to promoters. Proteasomal ATPases genetically interact with SAGA, and their inhibition reduces global histone H3 acetylation levels and SAGA recruitment to target promoters in vivo. These results indicate that the 19S RP modulates SAGA complex using its ATPase components, thereby facilitating subsequent transcription events at promoters.

AB - Promoter recruitment of the Saccharomyces cerevisiae SAGA histone acetyltransferase complex is required for RNA polymerase II-dependent transcription of several genes. SAGA is targeted to promoters through interactions with sequence-specific DNA binding transcriptional activators and facilitates preinitiation-complex assembly and transcription. Here, we show that the 19S proteasome regulatory particle (19S RP) alters SAGA to stimulate its interaction with transcriptional activators. The ATPase components of the 19S RP are required for stimulation of SAGA/activator interactions and enhance SAGA recruitment to promoters. Proteasomal ATPases genetically interact with SAGA, and their inhibition reduces global histone H3 acetylation levels and SAGA recruitment to target promoters in vivo. These results indicate that the 19S RP modulates SAGA complex using its ATPase components, thereby facilitating subsequent transcription events at promoters.

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JO - Cell

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The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators

Abstract

ASJC Scopus subject areas

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