The protective effects of histone deacetylases inhibitor TSA on the mice model of rheumatoid arthritis

Xin Hua, Yong Hua Bian, Xiao Lei Sun, Yu Hong Ji, Jie Zhang, Xiao Ying Wang, Xiao Rong Zhou

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the effects of trichostatin A (TSA) on the mice model of collagen induced arthritis(CIA). Methods: Mice model of rheumatoid arthritis(RA) was induced in DBA/1 mice with type II collagen. Paws were scored for histological severity of arthritis. The severity of inflammation of mouse joint was evaluated by histological examination. Real-time PCR was used to determine the cytokine mRNA expression. Cytokine production was measured by ELISA from serum, spleen cell culture or dendritic cell and T cell co-culture supernatant. T cell proliferation was examined by MTT method. Results: TSA can significantly suppress the severity of the arthritis in CIA. IFN-γ was elevated in CIA mice, but was inhibited significantly by TSA introduced either at the same time with immunization or at the onset of manifestation of arthritis. Collagen specific T cell proliferation was significantly suppressed by introduction of TSA. Increased level of IL-4 by T cells was observed in TSA treated group compared to that of control group. Conclusion: IL-4 level was increased and played a critical role in the protective effects of TSA in CIA. TSA suppresses the progress of CIA by regulates the balance of Th1/Th2 differentiation.

Original languageEnglish (US)
Pages (from-to)785-790
Number of pages6
JournalChinese Journal of Microbiology and Immunology
Volume30
Issue number9
DOIs
StatePublished - 2010

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trichostatin A
Histone Deacetylases
Experimental Arthritis
Rheumatoid Arthritis
Arthritis
T-Lymphocytes
Interleukin-4
Cell Culture Techniques
Cell Proliferation
Th1-Th2 Balance
Cytokines
Inbred DBA Mouse
Collagen Type II
Coculture Techniques
Dendritic Cells
Real-Time Polymerase Chain Reaction
Immunization
Collagen
Spleen
Joints

Keywords

  • IFN-γ
  • IL-4
  • Rheumatoid arthritis
  • Trichostatin A (TSA)

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology

Cite this

The protective effects of histone deacetylases inhibitor TSA on the mice model of rheumatoid arthritis. / Hua, Xin; Bian, Yong Hua; Sun, Xiao Lei; Ji, Yu Hong; Zhang, Jie; Wang, Xiao Ying; Zhou, Xiao Rong.

In: Chinese Journal of Microbiology and Immunology, Vol. 30, No. 9, 2010, p. 785-790.

Research output: Contribution to journalArticle

Hua, Xin ; Bian, Yong Hua ; Sun, Xiao Lei ; Ji, Yu Hong ; Zhang, Jie ; Wang, Xiao Ying ; Zhou, Xiao Rong. / The protective effects of histone deacetylases inhibitor TSA on the mice model of rheumatoid arthritis. In: Chinese Journal of Microbiology and Immunology. 2010 ; Vol. 30, No. 9. pp. 785-790.
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abstract = "Objective: To investigate the effects of trichostatin A (TSA) on the mice model of collagen induced arthritis(CIA). Methods: Mice model of rheumatoid arthritis(RA) was induced in DBA/1 mice with type II collagen. Paws were scored for histological severity of arthritis. The severity of inflammation of mouse joint was evaluated by histological examination. Real-time PCR was used to determine the cytokine mRNA expression. Cytokine production was measured by ELISA from serum, spleen cell culture or dendritic cell and T cell co-culture supernatant. T cell proliferation was examined by MTT method. Results: TSA can significantly suppress the severity of the arthritis in CIA. IFN-γ was elevated in CIA mice, but was inhibited significantly by TSA introduced either at the same time with immunization or at the onset of manifestation of arthritis. Collagen specific T cell proliferation was significantly suppressed by introduction of TSA. Increased level of IL-4 by T cells was observed in TSA treated group compared to that of control group. Conclusion: IL-4 level was increased and played a critical role in the protective effects of TSA in CIA. TSA suppresses the progress of CIA by regulates the balance of Th1/Th2 differentiation.",
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