The RAD2 gene of Saccharomyces cerevisiae: nucleotide sequence and transcript mapping

Charles M. Nicolet, Janet M. Chenevert, Errol C. Friedberg

Research output: Contribution to journalArticle

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Abstract

We have determined the nucleotide (nt) sequence of a segment of the yeast chromosome carrying the RAD2 gene. The coding region consists of 2925 bp and could encode a protein of 975 amino acids with a calculated Mr of 111 100. A major transcriptional start point was mapped to a position of approx. 22 bp upstream from the first ATG codon. A number of minor transcriptional start points were also identified in this region, all of them 5' to the putative translational start codon. We noted a number of consensus nt sequences in the 5' and 3 ' non-coding regions of the RAD2, RAD1 and RAD3 genes of Saccharomyces cerevisiae. In addition, three regions of amino acid sequence homology in the putative RAD1 and RAD2 polypeptides were observed.

Original languageEnglish (US)
Pages (from-to)225-234
Number of pages10
JournalGene
Volume36
Issue number3
DOIs
Publication statusPublished - 1985

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Keywords

  • cloning vector
  • DNA incision
  • DNA repair
  • Recombinant DNA
  • start point of mRNA
  • UV radiation
  • yeast

ASJC Scopus subject areas

  • Genetics

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