The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice.

Robert E Hammer, Galvin H Swift, D. M. Ornitz, C. J. Quaife, R. D. Palmiter, R. L. Brinster, Raymond J MacDonald

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

A total of 134 base pairs of the 5' flanking sequence of the elastase I gene is sufficient and necessary to direct expression of the passive human growth hormone gene (hGH) to the exocrine pancreas. We demonstrate that this elastase I regulatory region contains a transcriptional enhancer which directs acinar cell-specific expression in transgenic animals. The elastase I enhancer specifies correct expression of the linked hGH gene in an orientation- and position-independent manner and can activate a heterologous promoter. The enhancer also directs the appropriate temporal activation of the hGH gene in the developing pancreas. Transcription is initiated correctly for the elastase I or hGH promoter, and the transcripts are correctly processed regardless of the enhancer position within or outside the fusion gene. The elastase I enhancer generates coincident DNase I-hypersensitive sites in pancreatic chromatin when moved 3 kilobases upstream or within the first intron of the hGH gene and when associated with the hGH promoter.

Original languageEnglish (US)
Pages (from-to)2956-2967
Number of pages12
JournalMolecular and Cellular Biology
Volume7
Issue number8
StatePublished - Aug 1987

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Pancreatic Elastase
Transgenic Mice
Human Growth Hormone
Genes
Exocrine Pancreas
Genetically Modified Animals
Acinar Cells
5' Flanking Region
Nucleic Acid Regulatory Sequences
Gene Fusion
Deoxyribonuclease I
Base Pairing
Introns
Chromatin
Pancreas

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice. / Hammer, Robert E; Swift, Galvin H; Ornitz, D. M.; Quaife, C. J.; Palmiter, R. D.; Brinster, R. L.; MacDonald, Raymond J.

In: Molecular and Cellular Biology, Vol. 7, No. 8, 08.1987, p. 2956-2967.

Research output: Contribution to journalArticle

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AU - Brinster, R. L.

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