The rate of protein synthesis in hematopoietic stem cells is limited partly by 4E-BPs

Robert A J Signer, Le Qi, Zhiyu Zhao, David Thompson, Alla A. Sigova, Zi Peng Fan, George N. Demartino, Richard A. Young, Nahum Sonenberg, Sean J. Morrison

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Adult stem cells must limit their rate of protein synthesis, but the underlying mechanisms remain largely unexplored. Differences in protein synthesis among hematopoietic stem cells (HSCs) and progenitor cells did not correlate with differences in proteasome activity, total RNA content, mRNA content, or cell division rate. However, adult HSCs had more hypophosphorylated eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and 4E-BP2 as compared with most other hematopoietic progenitors. Deficiency for 4E-BP1 and 4E-BP2 significantly increased global protein synthesis in HSCs, but not in other hematopoietic progenitors, and impaired their reconstituting activity, identifying a mechanism that promotes HSC maintenance by attenuating protein synthesis.

Original languageEnglish (US)
Pages (from-to)1698-1703
Number of pages6
JournalGenes and Development
Volume30
Issue number15
DOIs
StatePublished - Aug 1 2016

Fingerprint

Hematopoietic Stem Cells
Adult Stem Cells
Carrier Proteins
Proteins
Eukaryotic Initiation Factor-4E
Proteasome Endopeptidase Complex
Cell Division
Stem Cells
Maintenance
RNA
Messenger RNA

Keywords

  • 4E-BP
  • Protein synthesis
  • Stem cell

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

The rate of protein synthesis in hematopoietic stem cells is limited partly by 4E-BPs. / Signer, Robert A J; Qi, Le; Zhao, Zhiyu; Thompson, David; Sigova, Alla A.; Fan, Zi Peng; Demartino, George N.; Young, Richard A.; Sonenberg, Nahum; Morrison, Sean J.

In: Genes and Development, Vol. 30, No. 15, 01.08.2016, p. 1698-1703.

Research output: Contribution to journalArticle

Signer, RAJ, Qi, L, Zhao, Z, Thompson, D, Sigova, AA, Fan, ZP, Demartino, GN, Young, RA, Sonenberg, N & Morrison, SJ 2016, 'The rate of protein synthesis in hematopoietic stem cells is limited partly by 4E-BPs', Genes and Development, vol. 30, no. 15, pp. 1698-1703. https://doi.org/10.1101/gad.282756.116
Signer, Robert A J ; Qi, Le ; Zhao, Zhiyu ; Thompson, David ; Sigova, Alla A. ; Fan, Zi Peng ; Demartino, George N. ; Young, Richard A. ; Sonenberg, Nahum ; Morrison, Sean J. / The rate of protein synthesis in hematopoietic stem cells is limited partly by 4E-BPs. In: Genes and Development. 2016 ; Vol. 30, No. 15. pp. 1698-1703.
@article{9a3274971e2d4c4b9ba9588a0b3e8d99,
title = "The rate of protein synthesis in hematopoietic stem cells is limited partly by 4E-BPs",
abstract = "Adult stem cells must limit their rate of protein synthesis, but the underlying mechanisms remain largely unexplored. Differences in protein synthesis among hematopoietic stem cells (HSCs) and progenitor cells did not correlate with differences in proteasome activity, total RNA content, mRNA content, or cell division rate. However, adult HSCs had more hypophosphorylated eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and 4E-BP2 as compared with most other hematopoietic progenitors. Deficiency for 4E-BP1 and 4E-BP2 significantly increased global protein synthesis in HSCs, but not in other hematopoietic progenitors, and impaired their reconstituting activity, identifying a mechanism that promotes HSC maintenance by attenuating protein synthesis.",
keywords = "4E-BP, Protein synthesis, Stem cell",
author = "Signer, {Robert A J} and Le Qi and Zhiyu Zhao and David Thompson and Sigova, {Alla A.} and Fan, {Zi Peng} and Demartino, {George N.} and Young, {Richard A.} and Nahum Sonenberg and Morrison, {Sean J.}",
year = "2016",
month = "8",
day = "1",
doi = "10.1101/gad.282756.116",
language = "English (US)",
volume = "30",
pages = "1698--1703",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "15",

}

TY - JOUR

T1 - The rate of protein synthesis in hematopoietic stem cells is limited partly by 4E-BPs

AU - Signer, Robert A J

AU - Qi, Le

AU - Zhao, Zhiyu

AU - Thompson, David

AU - Sigova, Alla A.

AU - Fan, Zi Peng

AU - Demartino, George N.

AU - Young, Richard A.

AU - Sonenberg, Nahum

AU - Morrison, Sean J.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Adult stem cells must limit their rate of protein synthesis, but the underlying mechanisms remain largely unexplored. Differences in protein synthesis among hematopoietic stem cells (HSCs) and progenitor cells did not correlate with differences in proteasome activity, total RNA content, mRNA content, or cell division rate. However, adult HSCs had more hypophosphorylated eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and 4E-BP2 as compared with most other hematopoietic progenitors. Deficiency for 4E-BP1 and 4E-BP2 significantly increased global protein synthesis in HSCs, but not in other hematopoietic progenitors, and impaired their reconstituting activity, identifying a mechanism that promotes HSC maintenance by attenuating protein synthesis.

AB - Adult stem cells must limit their rate of protein synthesis, but the underlying mechanisms remain largely unexplored. Differences in protein synthesis among hematopoietic stem cells (HSCs) and progenitor cells did not correlate with differences in proteasome activity, total RNA content, mRNA content, or cell division rate. However, adult HSCs had more hypophosphorylated eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and 4E-BP2 as compared with most other hematopoietic progenitors. Deficiency for 4E-BP1 and 4E-BP2 significantly increased global protein synthesis in HSCs, but not in other hematopoietic progenitors, and impaired their reconstituting activity, identifying a mechanism that promotes HSC maintenance by attenuating protein synthesis.

KW - 4E-BP

KW - Protein synthesis

KW - Stem cell

UR - http://www.scopus.com/inward/record.url?scp=84983337909&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84983337909&partnerID=8YFLogxK

U2 - 10.1101/gad.282756.116

DO - 10.1101/gad.282756.116

M3 - Article

VL - 30

SP - 1698

EP - 1703

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 15

ER -