The reaction of arachidonic acid epoxides (epoxyeicosatrienoic acids) with a cytosolic epoxide hydrolase

N. Chacos, J. Capdevila, J. R. Falck, S. Manna, C. Martin-Wixtrom, S. S. Gill, B. D. Hammock, R. W. Estabrook

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

Epoxyeicosatrienoic acids, formed during the cytochrome P-450-catalyzed oxidation of arachidonic acid, react with a liver cytosolic epoxide hydrolase to form vicinal diols of eicosatrienoic acid. The role of this cytosolic enzyme, rather than a microsomal bound type, explains previous results illustrating the ability to accumulate epoxides during the in vitro aerobic steady state of oxidative metabolism of arachidonic acid by liver microsomes. The inability of the 5,6-epoxyeicosatrienoic acid to serve as a suitable substrate for this enzyme is discussed in light of recent studies concerning possible unique physiological functions for this metabolite.

Original languageEnglish (US)
Pages (from-to)639-648
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume223
Issue number2
DOIs
StatePublished - 1983

Fingerprint

Epoxide Hydrolases
Epoxy Compounds
Arachidonic Acid
Liver
Acids
Liver Microsomes
Enzymes
Metabolites
Metabolism
Cytochrome P-450 Enzyme System
Oxidation
Substrates
5,6-epoxy-8,11,14-eicosatrienoic acid
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The reaction of arachidonic acid epoxides (epoxyeicosatrienoic acids) with a cytosolic epoxide hydrolase. / Chacos, N.; Capdevila, J.; Falck, J. R.; Manna, S.; Martin-Wixtrom, C.; Gill, S. S.; Hammock, B. D.; Estabrook, R. W.

In: Archives of Biochemistry and Biophysics, Vol. 223, No. 2, 1983, p. 639-648.

Research output: Contribution to journalArticle

Chacos, N, Capdevila, J, Falck, JR, Manna, S, Martin-Wixtrom, C, Gill, SS, Hammock, BD & Estabrook, RW 1983, 'The reaction of arachidonic acid epoxides (epoxyeicosatrienoic acids) with a cytosolic epoxide hydrolase', Archives of Biochemistry and Biophysics, vol. 223, no. 2, pp. 639-648. https://doi.org/10.1016/0003-9861(83)90628-8
Chacos, N. ; Capdevila, J. ; Falck, J. R. ; Manna, S. ; Martin-Wixtrom, C. ; Gill, S. S. ; Hammock, B. D. ; Estabrook, R. W. / The reaction of arachidonic acid epoxides (epoxyeicosatrienoic acids) with a cytosolic epoxide hydrolase. In: Archives of Biochemistry and Biophysics. 1983 ; Vol. 223, No. 2. pp. 639-648.
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