The reaction of arachidonic acid epoxides (epoxyeicosatrienoic acids) with a cytosolic epoxide hydrolase

N. Chacos; J. Capdevila; J. R. Falck; S. Manna; C. Martin-Wixtrom; S. S. Gill; B. D. Hammock; R. W. Estabrook

doi:10.1016/0003-9861(83)90628-8

The reaction of arachidonic acid epoxides (epoxyeicosatrienoic acids) with a cytosolic epoxide hydrolase

N. Chacos, J. Capdevila, J. R. Falck, S. Manna, C. Martin-Wixtrom, S. S. Gill, B. D. Hammock, R. W. Estabrook

Biochemistry

Research output: Contribution to journal › Article

165 Scopus citations

Abstract

Epoxyeicosatrienoic acids, formed during the cytochrome P-450-catalyzed oxidation of arachidonic acid, react with a liver cytosolic epoxide hydrolase to form vicinal diols of eicosatrienoic acid. The role of this cytosolic enzyme, rather than a microsomal bound type, explains previous results illustrating the ability to accumulate epoxides during the in vitro aerobic steady state of oxidative metabolism of arachidonic acid by liver microsomes. The inability of the 5,6-epoxyeicosatrienoic acid to serve as a suitable substrate for this enzyme is discussed in light of recent studies concerning possible unique physiological functions for this metabolite.

Original language	English (US)
Pages (from-to)	639-648
Number of pages	10
Journal	Archives of Biochemistry and Biophysics
Volume	223
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(83)90628-8
State	Published - Jun 1983

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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Chacos, N., Capdevila, J., Falck, J. R., Manna, S., Martin-Wixtrom, C., Gill, S. S., Hammock, B. D., & Estabrook, R. W. (1983). The reaction of arachidonic acid epoxides (epoxyeicosatrienoic acids) with a cytosolic epoxide hydrolase. Archives of Biochemistry and Biophysics, 223(2), 639-648. https://doi.org/10.1016/0003-9861(83)90628-8

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abstract = "Epoxyeicosatrienoic acids, formed during the cytochrome P-450-catalyzed oxidation of arachidonic acid, react with a liver cytosolic epoxide hydrolase to form vicinal diols of eicosatrienoic acid. The role of this cytosolic enzyme, rather than a microsomal bound type, explains previous results illustrating the ability to accumulate epoxides during the in vitro aerobic steady state of oxidative metabolism of arachidonic acid by liver microsomes. The inability of the 5,6-epoxyeicosatrienoic acid to serve as a suitable substrate for this enzyme is discussed in light of recent studies concerning possible unique physiological functions for this metabolite.",

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AU - Capdevila, J.

AU - Falck, J. R.

AU - Manna, S.

AU - Martin-Wixtrom, C.

AU - Gill, S. S.

AU - Hammock, B. D.

AU - Estabrook, R. W.

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AB - Epoxyeicosatrienoic acids, formed during the cytochrome P-450-catalyzed oxidation of arachidonic acid, react with a liver cytosolic epoxide hydrolase to form vicinal diols of eicosatrienoic acid. The role of this cytosolic enzyme, rather than a microsomal bound type, explains previous results illustrating the ability to accumulate epoxides during the in vitro aerobic steady state of oxidative metabolism of arachidonic acid by liver microsomes. The inability of the 5,6-epoxyeicosatrienoic acid to serve as a suitable substrate for this enzyme is discussed in light of recent studies concerning possible unique physiological functions for this metabolite.

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