The renal nerve is required for regulation of proximal tubule transport by intraluminally produced ANG II

Albert Quan, Michel Baum

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25 Citations (Scopus)

Abstract

The proximal tubule synthesizes and luminally secretes high levels of angiotensin II, which modulate proximal tubule transport independently of systemic angiotensin II. The purpose of this in vivo microperfusion study is to examine whether the renal nerves modulate the effect of intraluminal angiotensin II on proximal tubule transport. The decrement in volume reabsorption after addition of 10-4 M luminal enalaprilat is a measure of the role of luminal angiotensin II on transport. Acute denervation decreased volume reabsorption (2.97 ± 0.14 vs. 1.30 ± 0.21 nl·mm-1·min-1, P < 0.001). Although luminal 10-4 M enalaprilat decreased volume reabsorption in controls (2.97 ± 0.14 vs. 1.61 ± 0.26 nl·mm-1·min-1, P < 0.001), it did not after acute denervation (1.30 ± 0.21 vs. 1.55 ± 0.19 nl·mm-1·min-1). After chronic denervation, volume reabsorption was unchanged from sham controls (2.26 ± 0.28 vs. 2.70 ± 0.19 nl·mm-1·min-1). Addition of luminal 10-4 M enalaprilat decreased volume reabsorption in sham control (2.70 ± 0.19 vs. 1.60 ± 0.10 nl·mm-1·min-1, P < 0.05) but not with chronic denervation (2.26 ± 0.28 vs. 2.07 ± 0.20 nl·mm-1·min-1). Addition of 10-8 M angiotensin II to the lumen does not affect transport due to the presence of luminal angiotensin II. However, addition of 10-8 M angiotensin II to the tubular lumen increased the volume reabsorption after both acute (1.30 ± 0.21 vs. 2.67 ± 0.18 nl·mm-1·min-1, P < 0.05) and chronic denervation (2.26 ± 0.28 vs. 3.57 ± 0.44 nl·mm-1·min-1, P < 0.01). These data indicate that renal denervation abolished the luminal enalaprilat-sensitive component of proximal tubule transport, which is consistent with the renal nerves playing a role in the modulation of the intraluminal angiotensin II mediated component of proximal tubule transport.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume280
Issue number3 49-3
StatePublished - 2001

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Angiotensin II
Denervation
Enalaprilat
Kidney

Keywords

  • Renal denervation
  • Renin-angiotensin system
  • Volume reabsorption

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

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title = "The renal nerve is required for regulation of proximal tubule transport by intraluminally produced ANG II",
abstract = "The proximal tubule synthesizes and luminally secretes high levels of angiotensin II, which modulate proximal tubule transport independently of systemic angiotensin II. The purpose of this in vivo microperfusion study is to examine whether the renal nerves modulate the effect of intraluminal angiotensin II on proximal tubule transport. The decrement in volume reabsorption after addition of 10-4 M luminal enalaprilat is a measure of the role of luminal angiotensin II on transport. Acute denervation decreased volume reabsorption (2.97 ± 0.14 vs. 1.30 ± 0.21 nl·mm-1·min-1, P < 0.001). Although luminal 10-4 M enalaprilat decreased volume reabsorption in controls (2.97 ± 0.14 vs. 1.61 ± 0.26 nl·mm-1·min-1, P < 0.001), it did not after acute denervation (1.30 ± 0.21 vs. 1.55 ± 0.19 nl·mm-1·min-1). After chronic denervation, volume reabsorption was unchanged from sham controls (2.26 ± 0.28 vs. 2.70 ± 0.19 nl·mm-1·min-1). Addition of luminal 10-4 M enalaprilat decreased volume reabsorption in sham control (2.70 ± 0.19 vs. 1.60 ± 0.10 nl·mm-1·min-1, P < 0.05) but not with chronic denervation (2.26 ± 0.28 vs. 2.07 ± 0.20 nl·mm-1·min-1). Addition of 10-8 M angiotensin II to the lumen does not affect transport due to the presence of luminal angiotensin II. However, addition of 10-8 M angiotensin II to the tubular lumen increased the volume reabsorption after both acute (1.30 ± 0.21 vs. 2.67 ± 0.18 nl·mm-1·min-1, P < 0.05) and chronic denervation (2.26 ± 0.28 vs. 3.57 ± 0.44 nl·mm-1·min-1, P < 0.01). These data indicate that renal denervation abolished the luminal enalaprilat-sensitive component of proximal tubule transport, which is consistent with the renal nerves playing a role in the modulation of the intraluminal angiotensin II mediated component of proximal tubule transport.",
keywords = "Renal denervation, Renin-angiotensin system, Volume reabsorption",
author = "Albert Quan and Michel Baum",
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T1 - The renal nerve is required for regulation of proximal tubule transport by intraluminally produced ANG II

AU - Quan, Albert

AU - Baum, Michel

PY - 2001

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N2 - The proximal tubule synthesizes and luminally secretes high levels of angiotensin II, which modulate proximal tubule transport independently of systemic angiotensin II. The purpose of this in vivo microperfusion study is to examine whether the renal nerves modulate the effect of intraluminal angiotensin II on proximal tubule transport. The decrement in volume reabsorption after addition of 10-4 M luminal enalaprilat is a measure of the role of luminal angiotensin II on transport. Acute denervation decreased volume reabsorption (2.97 ± 0.14 vs. 1.30 ± 0.21 nl·mm-1·min-1, P < 0.001). Although luminal 10-4 M enalaprilat decreased volume reabsorption in controls (2.97 ± 0.14 vs. 1.61 ± 0.26 nl·mm-1·min-1, P < 0.001), it did not after acute denervation (1.30 ± 0.21 vs. 1.55 ± 0.19 nl·mm-1·min-1). After chronic denervation, volume reabsorption was unchanged from sham controls (2.26 ± 0.28 vs. 2.70 ± 0.19 nl·mm-1·min-1). Addition of luminal 10-4 M enalaprilat decreased volume reabsorption in sham control (2.70 ± 0.19 vs. 1.60 ± 0.10 nl·mm-1·min-1, P < 0.05) but not with chronic denervation (2.26 ± 0.28 vs. 2.07 ± 0.20 nl·mm-1·min-1). Addition of 10-8 M angiotensin II to the lumen does not affect transport due to the presence of luminal angiotensin II. However, addition of 10-8 M angiotensin II to the tubular lumen increased the volume reabsorption after both acute (1.30 ± 0.21 vs. 2.67 ± 0.18 nl·mm-1·min-1, P < 0.05) and chronic denervation (2.26 ± 0.28 vs. 3.57 ± 0.44 nl·mm-1·min-1, P < 0.01). These data indicate that renal denervation abolished the luminal enalaprilat-sensitive component of proximal tubule transport, which is consistent with the renal nerves playing a role in the modulation of the intraluminal angiotensin II mediated component of proximal tubule transport.

AB - The proximal tubule synthesizes and luminally secretes high levels of angiotensin II, which modulate proximal tubule transport independently of systemic angiotensin II. The purpose of this in vivo microperfusion study is to examine whether the renal nerves modulate the effect of intraluminal angiotensin II on proximal tubule transport. The decrement in volume reabsorption after addition of 10-4 M luminal enalaprilat is a measure of the role of luminal angiotensin II on transport. Acute denervation decreased volume reabsorption (2.97 ± 0.14 vs. 1.30 ± 0.21 nl·mm-1·min-1, P < 0.001). Although luminal 10-4 M enalaprilat decreased volume reabsorption in controls (2.97 ± 0.14 vs. 1.61 ± 0.26 nl·mm-1·min-1, P < 0.001), it did not after acute denervation (1.30 ± 0.21 vs. 1.55 ± 0.19 nl·mm-1·min-1). After chronic denervation, volume reabsorption was unchanged from sham controls (2.26 ± 0.28 vs. 2.70 ± 0.19 nl·mm-1·min-1). Addition of luminal 10-4 M enalaprilat decreased volume reabsorption in sham control (2.70 ± 0.19 vs. 1.60 ± 0.10 nl·mm-1·min-1, P < 0.05) but not with chronic denervation (2.26 ± 0.28 vs. 2.07 ± 0.20 nl·mm-1·min-1). Addition of 10-8 M angiotensin II to the lumen does not affect transport due to the presence of luminal angiotensin II. However, addition of 10-8 M angiotensin II to the tubular lumen increased the volume reabsorption after both acute (1.30 ± 0.21 vs. 2.67 ± 0.18 nl·mm-1·min-1, P < 0.05) and chronic denervation (2.26 ± 0.28 vs. 3.57 ± 0.44 nl·mm-1·min-1, P < 0.01). These data indicate that renal denervation abolished the luminal enalaprilat-sensitive component of proximal tubule transport, which is consistent with the renal nerves playing a role in the modulation of the intraluminal angiotensin II mediated component of proximal tubule transport.

KW - Renal denervation

KW - Renin-angiotensin system

KW - Volume reabsorption

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