The role of cytokines in polymyositis

III. Recombinant human interferon-γ enhances T cell adhesion to cultured human muscle cells

Apostolos E. Kalovidouris, Claire A. Horn, Zoya Plotkin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective. To investigate the effect of interferon-γ (IFNγ) on the adhesive interactions between human peripheral blood T cells and human skeletal muscle cells at various stages of muscle cell differentiation and maturation in vitro. Methods. Human muscle cell cultures were established from normal muscle biopsy material, using the explant technique. T cells were studied for their capacity to adhere to IFNγ-treated and untreated myoblasts and myotubes. The role of intercellular adhesion molecule type 1 (ICAM-1) in cell adhesion to muscle cells was examined in blocking studies, by enzyme-linked immunosorbent assay (ELISA), and by immunohistochemical staining using monoclonal antibodies (MAb). Results. Treatment of muscle cells (myoblasts and myotubes) with IFNγ resulted in a significant dose-dependent increase in the number of adherent T cells. Adhesion of T cells to muscle cells was significantly inhibited by MAb to ICAM-1 and to lymphocyte function-associated antigen type 1, but not by MAb to HLA-DR. There was no difference in the level of T cell adhesion to IFNγ-treated allogeneic versus autologous muscle cells. By ELISA and immunohistochemical analysis, ICAM-1 expression on the surface of cultured human muscle cells was either absent or barely detectable, but was strongly induced by treatment of muscle cells with IFNγ. Conclusion. Induction of cell adhesion molecules on muscle cells by IFNγ may be an important mechanism for the localization of T cells in the affected muscles of patients with autoimmune myositis.

Original languageEnglish (US)
Pages (from-to)907-914
Number of pages8
JournalArthritis and Rheumatism
Volume37
Issue number6
StatePublished - Jun 1994

Fingerprint

Polymyositis
Cell Adhesion
Muscle Cells
Interferons
Cytokines
T-Lymphocytes
Intercellular Adhesion Molecule-1
Myoblasts
Skeletal Muscle Fibers
Monoclonal Antibodies
Enzyme-Linked Immunosorbent Assay
Muscles
Lymphocyte Function-Associated Antigen-1
Myositis
Cell Adhesion Molecules
HLA-DR Antigens
Adhesives
Cell Differentiation
Blood Cells
Skeletal Muscle

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

The role of cytokines in polymyositis : III. Recombinant human interferon-γ enhances T cell adhesion to cultured human muscle cells. / Kalovidouris, Apostolos E.; Horn, Claire A.; Plotkin, Zoya.

In: Arthritis and Rheumatism, Vol. 37, No. 6, 06.1994, p. 907-914.

Research output: Contribution to journalArticle

Kalovidouris, Apostolos E. ; Horn, Claire A. ; Plotkin, Zoya. / The role of cytokines in polymyositis : III. Recombinant human interferon-γ enhances T cell adhesion to cultured human muscle cells. In: Arthritis and Rheumatism. 1994 ; Vol. 37, No. 6. pp. 907-914.
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