The role of low density, high density, and very low density lipoproteins in steroidogenesis by the human fetal adrenal gland

B. R. Carr, C. R. Parker, L. Milewich, J. C. Porter, P. C. MacDonald, E. R. Simpson

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

In the present investigation the role of serum lipoproteins in fetal adrenal steroidogenesis was evaluated. Human fetal adrenal tissue was maintained in organ culture for 6 days in the presence or absence of ACTH. The culture medium contained either human serum (10%, vol/vol) or lipoprotein-poor serum (10%, vol/vol) with or without low density lipoprotein (LDL), high density lipoprotein, or very low density lipoprotein. The medium was changed daily and assayed for pregnenolone sulfate, dehydroepiandrosterone sulfate (DS), and cortisol. In the absence of ACTH, steroid secretion decreased to negligible rates, regardless of the presence or absence of lipoprotein in the culture medium. However, the cumulative secretions of DS, pregnenolone sulfate, and cortisol for the entire 6-day culture period in the presence of ACTH plus optimal amounts of LDL or human serum were 72.5 and 72.2 μg/mg tissue protein, respectively. These values were greater than those observed in the absence of lipoproteins (37.6 μg/mg tissue protein) or when very low density or high density lipoprotein was included in the medium (35.2 and 54.0 μg/mg protein, respectively). When various concentrations of LDL were employed, maximal secretion rates for DS and cortisol were observed when the concentration of LDL protein was 150-200 μg/ml. It is concluded that plasma lipoprotein cholesterol is used by fetal adrenal tissue in culture for steroidogenesis, and that LDL appears to be the lipoprotein preferred as a source of cholesterol for steroidogenesis by this tissue.

Original languageEnglish (US)
Pages (from-to)1854-1860
Number of pages7
JournalEndocrinology
Volume106
Issue number6
StatePublished - 1980

Fingerprint

VLDL Lipoproteins
Adrenal Glands
LDL Lipoproteins
Lipoproteins
Dehydroepiandrosterone Sulfate
Adrenocorticotropic Hormone
Hydrocortisone
HDL Lipoproteins
Serum
Culture Media
Proteins
Fetus
Organ Culture Techniques
Steroids
Cholesterol
pregnenolone sulfate

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Carr, B. R., Parker, C. R., Milewich, L., Porter, J. C., MacDonald, P. C., & Simpson, E. R. (1980). The role of low density, high density, and very low density lipoproteins in steroidogenesis by the human fetal adrenal gland. Endocrinology, 106(6), 1854-1860.

The role of low density, high density, and very low density lipoproteins in steroidogenesis by the human fetal adrenal gland. / Carr, B. R.; Parker, C. R.; Milewich, L.; Porter, J. C.; MacDonald, P. C.; Simpson, E. R.

In: Endocrinology, Vol. 106, No. 6, 1980, p. 1854-1860.

Research output: Contribution to journalArticle

Carr, BR, Parker, CR, Milewich, L, Porter, JC, MacDonald, PC & Simpson, ER 1980, 'The role of low density, high density, and very low density lipoproteins in steroidogenesis by the human fetal adrenal gland', Endocrinology, vol. 106, no. 6, pp. 1854-1860.
Carr, B. R. ; Parker, C. R. ; Milewich, L. ; Porter, J. C. ; MacDonald, P. C. ; Simpson, E. R. / The role of low density, high density, and very low density lipoproteins in steroidogenesis by the human fetal adrenal gland. In: Endocrinology. 1980 ; Vol. 106, No. 6. pp. 1854-1860.
@article{f2a3ae6e25a942848f67a02f15835e99,
title = "The role of low density, high density, and very low density lipoproteins in steroidogenesis by the human fetal adrenal gland",
abstract = "In the present investigation the role of serum lipoproteins in fetal adrenal steroidogenesis was evaluated. Human fetal adrenal tissue was maintained in organ culture for 6 days in the presence or absence of ACTH. The culture medium contained either human serum (10{\%}, vol/vol) or lipoprotein-poor serum (10{\%}, vol/vol) with or without low density lipoprotein (LDL), high density lipoprotein, or very low density lipoprotein. The medium was changed daily and assayed for pregnenolone sulfate, dehydroepiandrosterone sulfate (DS), and cortisol. In the absence of ACTH, steroid secretion decreased to negligible rates, regardless of the presence or absence of lipoprotein in the culture medium. However, the cumulative secretions of DS, pregnenolone sulfate, and cortisol for the entire 6-day culture period in the presence of ACTH plus optimal amounts of LDL or human serum were 72.5 and 72.2 μg/mg tissue protein, respectively. These values were greater than those observed in the absence of lipoproteins (37.6 μg/mg tissue protein) or when very low density or high density lipoprotein was included in the medium (35.2 and 54.0 μg/mg protein, respectively). When various concentrations of LDL were employed, maximal secretion rates for DS and cortisol were observed when the concentration of LDL protein was 150-200 μg/ml. It is concluded that plasma lipoprotein cholesterol is used by fetal adrenal tissue in culture for steroidogenesis, and that LDL appears to be the lipoprotein preferred as a source of cholesterol for steroidogenesis by this tissue.",
author = "Carr, {B. R.} and Parker, {C. R.} and L. Milewich and Porter, {J. C.} and MacDonald, {P. C.} and Simpson, {E. R.}",
year = "1980",
language = "English (US)",
volume = "106",
pages = "1854--1860",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "The Endocrine Society",
number = "6",

}

TY - JOUR

T1 - The role of low density, high density, and very low density lipoproteins in steroidogenesis by the human fetal adrenal gland

AU - Carr, B. R.

AU - Parker, C. R.

AU - Milewich, L.

AU - Porter, J. C.

AU - MacDonald, P. C.

AU - Simpson, E. R.

PY - 1980

Y1 - 1980

N2 - In the present investigation the role of serum lipoproteins in fetal adrenal steroidogenesis was evaluated. Human fetal adrenal tissue was maintained in organ culture for 6 days in the presence or absence of ACTH. The culture medium contained either human serum (10%, vol/vol) or lipoprotein-poor serum (10%, vol/vol) with or without low density lipoprotein (LDL), high density lipoprotein, or very low density lipoprotein. The medium was changed daily and assayed for pregnenolone sulfate, dehydroepiandrosterone sulfate (DS), and cortisol. In the absence of ACTH, steroid secretion decreased to negligible rates, regardless of the presence or absence of lipoprotein in the culture medium. However, the cumulative secretions of DS, pregnenolone sulfate, and cortisol for the entire 6-day culture period in the presence of ACTH plus optimal amounts of LDL or human serum were 72.5 and 72.2 μg/mg tissue protein, respectively. These values were greater than those observed in the absence of lipoproteins (37.6 μg/mg tissue protein) or when very low density or high density lipoprotein was included in the medium (35.2 and 54.0 μg/mg protein, respectively). When various concentrations of LDL were employed, maximal secretion rates for DS and cortisol were observed when the concentration of LDL protein was 150-200 μg/ml. It is concluded that plasma lipoprotein cholesterol is used by fetal adrenal tissue in culture for steroidogenesis, and that LDL appears to be the lipoprotein preferred as a source of cholesterol for steroidogenesis by this tissue.

AB - In the present investigation the role of serum lipoproteins in fetal adrenal steroidogenesis was evaluated. Human fetal adrenal tissue was maintained in organ culture for 6 days in the presence or absence of ACTH. The culture medium contained either human serum (10%, vol/vol) or lipoprotein-poor serum (10%, vol/vol) with or without low density lipoprotein (LDL), high density lipoprotein, or very low density lipoprotein. The medium was changed daily and assayed for pregnenolone sulfate, dehydroepiandrosterone sulfate (DS), and cortisol. In the absence of ACTH, steroid secretion decreased to negligible rates, regardless of the presence or absence of lipoprotein in the culture medium. However, the cumulative secretions of DS, pregnenolone sulfate, and cortisol for the entire 6-day culture period in the presence of ACTH plus optimal amounts of LDL or human serum were 72.5 and 72.2 μg/mg tissue protein, respectively. These values were greater than those observed in the absence of lipoproteins (37.6 μg/mg tissue protein) or when very low density or high density lipoprotein was included in the medium (35.2 and 54.0 μg/mg protein, respectively). When various concentrations of LDL were employed, maximal secretion rates for DS and cortisol were observed when the concentration of LDL protein was 150-200 μg/ml. It is concluded that plasma lipoprotein cholesterol is used by fetal adrenal tissue in culture for steroidogenesis, and that LDL appears to be the lipoprotein preferred as a source of cholesterol for steroidogenesis by this tissue.

UR - http://www.scopus.com/inward/record.url?scp=0018904270&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018904270&partnerID=8YFLogxK

M3 - Article

VL - 106

SP - 1854

EP - 1860

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -