The role of NADH-NADPH oxidase activity in the leukocyte function of burned patients

E. L. Heck, M. A. Edgar, B. S. Masters, C. R. Baxter

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Irreversible sepsis, in spite of advancements to topical therapy and antimicrobial agents, remains the leading cause of death in major thermal injury. A defect in intracellular bactericidal capacity in leukocytes from severely burned patients appears to correspond with increases in bacterial wound colonization and ultimate sepsis. This leukocyte defect has been demonstrated by abnormally low nitroblue tetrazolium reduction (NBT) and oxygen consumption of white cells in patients with major thermal injury. The subcellular mechanisms responsible for decreased bactericidal capacity were therefore investigated. Nicotinamide-adenine dinucleotide (NADH) and nicotinamide-adenine phosphodinucleotide (NADPH) oxidase activity was measured in patients with major burns, controls (normals), and in patients with nonburn stress or infection. NADH and NADPH oxidase levels in leukocytes from burn patients were not significantly different from those of normal nonchallenged controls but were significantly lower than the leukocyte values found in the patients with nonburn infections or stress. This NADH and NADPH defect in the subcellular leukocyte fraction suggests that it may be a significant factor in the reduced bactericidal function of the intact leukocyte in thermally injured patients.

Original languageEnglish (US)
Pages (from-to)49-51
Number of pages3
JournalJournal of Trauma
Volume19
Issue number1
StatePublished - 1979

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Niacinamide
Adenine
NAD
Oxidoreductases
Leukocytes
Sepsis
Wounds and Injuries
Hot Temperature
Nitroblue Tetrazolium
Subcellular Fractions
Anti-Infective Agents
Infection
Burns
Oxygen Consumption
Cause of Death

ASJC Scopus subject areas

  • Surgery

Cite this

Heck, E. L., Edgar, M. A., Masters, B. S., & Baxter, C. R. (1979). The role of NADH-NADPH oxidase activity in the leukocyte function of burned patients. Journal of Trauma, 19(1), 49-51.

The role of NADH-NADPH oxidase activity in the leukocyte function of burned patients. / Heck, E. L.; Edgar, M. A.; Masters, B. S.; Baxter, C. R.

In: Journal of Trauma, Vol. 19, No. 1, 1979, p. 49-51.

Research output: Contribution to journalArticle

Heck, EL, Edgar, MA, Masters, BS & Baxter, CR 1979, 'The role of NADH-NADPH oxidase activity in the leukocyte function of burned patients', Journal of Trauma, vol. 19, no. 1, pp. 49-51.
Heck, E. L. ; Edgar, M. A. ; Masters, B. S. ; Baxter, C. R. / The role of NADH-NADPH oxidase activity in the leukocyte function of burned patients. In: Journal of Trauma. 1979 ; Vol. 19, No. 1. pp. 49-51.
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