The role of Rab3A in neurotransmitter release

Martin Geppert, Vadim Y. Bolshakov, Steven A. Siegelbaum, Kohji Takei, Pietro De Camilli, Robert E Hammer, Thomas C. Südhof

Research output: Contribution to journalArticle

380 Citations (Scopus)

Abstract

THE small GTP-binding protein Rab3A is a Rab family member that is abundant in brain synaptic vesicles. Here we show that mice in which the rab3A gene has been mutated by homologous recombination do not express Rab3A but are viable and fertile. Electrophysiological recordings in hippocampal CA1 pyramidal cells indicate that most of their synaptic parameters are also normal, although synaptic depression after short trains of repetitive stimuli (15-30 stimuli at 14 Hz) is significantly increased. Levels of the Rab3A- binding protein rabphilin are decreased by 70%, but expression of more than 20 other synaptic proteins is unchanged. No compensatory changes were detected in other GTP-binding proteins or in proteins that interact with Rab3. Rab3A thus appears not to be essential for synaptic vesicle exocytosis but to play a role in the recruitment of synaptic vesicles for exocytosis during repetitive stimulation.

Original languageEnglish (US)
Pages (from-to)493-497
Number of pages5
JournalNature
Volume369
Issue number6480
DOIs
StatePublished - Jun 9 1994

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rab3A GTP-Binding Protein
Synaptic Vesicles
Neurotransmitter Agents
Exocytosis
Pyramidal Cells
Homologous Recombination
GTP-Binding Proteins
Carrier Proteins
Proteins
Brain
Genes

ASJC Scopus subject areas

  • General

Cite this

Geppert, M., Bolshakov, V. Y., Siegelbaum, S. A., Takei, K., De Camilli, P., Hammer, R. E., & Südhof, T. C. (1994). The role of Rab3A in neurotransmitter release. Nature, 369(6480), 493-497. https://doi.org/10.1038/369493a0

The role of Rab3A in neurotransmitter release. / Geppert, Martin; Bolshakov, Vadim Y.; Siegelbaum, Steven A.; Takei, Kohji; De Camilli, Pietro; Hammer, Robert E; Südhof, Thomas C.

In: Nature, Vol. 369, No. 6480, 09.06.1994, p. 493-497.

Research output: Contribution to journalArticle

Geppert, M, Bolshakov, VY, Siegelbaum, SA, Takei, K, De Camilli, P, Hammer, RE & Südhof, TC 1994, 'The role of Rab3A in neurotransmitter release', Nature, vol. 369, no. 6480, pp. 493-497. https://doi.org/10.1038/369493a0
Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE et al. The role of Rab3A in neurotransmitter release. Nature. 1994 Jun 9;369(6480):493-497. https://doi.org/10.1038/369493a0
Geppert, Martin ; Bolshakov, Vadim Y. ; Siegelbaum, Steven A. ; Takei, Kohji ; De Camilli, Pietro ; Hammer, Robert E ; Südhof, Thomas C. / The role of Rab3A in neurotransmitter release. In: Nature. 1994 ; Vol. 369, No. 6480. pp. 493-497.
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