The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo

Joachim Seemann, Eija Jämsä Jokitalo, Graham Warren

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Biochemical data have shown that COPI-coated vesicles are tethered to Golgi membranes by a complex of at least three proteins: p115, giantin, and GM130. p115 binds to giantin on the vesicles and to GM130 on the membrane. We now examine the function of this tethering complex in vivo. Microinjection of an N-terminal peptide of GM130 or overexpression of GM130 lacking this N-terminal peptide inhibits the binding of p115 to Golgi membranes. Electron microscopic analysis of single microinjected cells shows that the number of COP-sized transport vesicles in the Golgi region increases substantially, suggesting that transport vesicles continue to bud but are less able to fuse. This was corroborated by quantitative immunofluorescence analysis, which showed that the intracellular transport of the VSV-G protein was significantly inhibited. Together, these data suggest that this tethering complex increases the efficiency with which transport vesicles fuse with their target membrane. They also provide support for a model of mitotic Golgi fragmentation in which the tethering complex is disrupted by mitotic phosphorylation of GM130.

Original languageEnglish (US)
Pages (from-to)635-645
Number of pages11
JournalMolecular Biology of the Cell
Volume11
Issue number2
StatePublished - Feb 2000

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Golgi Apparatus
Transport Vesicles
Membranes
COP-Coated Vesicles
Proteins
Peptides
Microinjections
GTP-Binding Proteins
Fluorescent Antibody Technique
Cell Count
Phosphorylation
Electrons
macrogolgin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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The role of the tethering proteins p115 and GM130 in transport through the Golgi apparatus in vivo. / Seemann, Joachim; Jokitalo, Eija Jämsä; Warren, Graham.

In: Molecular Biology of the Cell, Vol. 11, No. 2, 02.2000, p. 635-645.

Research output: Contribution to journalArticle

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