The roles and mechanisms of homogalacturonan and rhamnogalacturonan I pectins on the inhibition of cell migration

Yuying Fan, Lin Sun, Siwen Yang, Congcong He, Guihua Tai, Yifa Zhou

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Our previous paper reported the structure of ginseng pectic polysaccharides related to the cell migration inhibitory effects, but the underlying mechanisms are poorly understood. In this manuscript, rhamnogalacturonan I (RGI)-rich pectins prepared from ginseng pectin were investigated for their effect on cell migration. The results indicated that the combination of homogalacturonan (HG) and RGI-rich pectins exerted stronger effects than either HG- or RGI-rich pectin alone. Further studies revealed that the effects of HG- and RGI-rich pectins were dependent on pretreatment, which caused alterations in cell morphologies such as cell size and shape, focal adhesion, and the organization of actin filaments, suggesting that HG and RGI pectins exert synergistic effects on cell migration, likely through different ways. Morphological data and quantitative cell adhesion and spreading assays showed that HG- and RGI-rich pectin treatment decreased cell adhesion and cell spreading on the substratum, suggesting that HG- and RGI-rich pectins may exert their effects on cell migration via decreasing cell adhesion and cell spreading. Additionally, we showed that L-929 cells expressed little galectin-3 (Gal-3) and that lactose, an inhibitor of Gal-3 did not block the activities of HG- and RGI-rich pectins, implicating that cell migration inhibited by pectin did not correlate to Gal-3.

Original languageEnglish (US)
Pages (from-to)207-217
Number of pages11
JournalInternational Journal of Biological Macromolecules
Volume106
DOIs
StatePublished - Jan 1 2018

Fingerprint

Cell Migration Inhibition
Pectins
Cells
Cell Movement
Galectin 3
Cell adhesion
Cell Adhesion
Panax
Focal Adhesions
polygalacturonic acid
rhamnogalacturonan I
Cell Shape
Lactose
Actin Cytoskeleton
Cell Size
Polysaccharides
Actins
Assays
Adhesion
pectin

Keywords

  • Cell migration
  • Galectin-3
  • Ginseng pectin
  • Homogalacturonan
  • Rhamnogalacturonan I

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

The roles and mechanisms of homogalacturonan and rhamnogalacturonan I pectins on the inhibition of cell migration. / Fan, Yuying; Sun, Lin; Yang, Siwen; He, Congcong; Tai, Guihua; Zhou, Yifa.

In: International Journal of Biological Macromolecules, Vol. 106, 01.01.2018, p. 207-217.

Research output: Contribution to journalArticle

Fan, Yuying ; Sun, Lin ; Yang, Siwen ; He, Congcong ; Tai, Guihua ; Zhou, Yifa. / The roles and mechanisms of homogalacturonan and rhamnogalacturonan I pectins on the inhibition of cell migration. In: International Journal of Biological Macromolecules. 2018 ; Vol. 106. pp. 207-217.
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