TY - JOUR
T1 - The roles and mechanisms of homogalacturonan and rhamnogalacturonan I pectins on the inhibition of cell migration
AU - Fan, Yuying
AU - Sun, Lin
AU - Yang, Siwen
AU - He, Congcong
AU - Tai, Guihua
AU - Zhou, Yifa
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China (Grant Nos. 31300287 and 31470798 ) and Fundamental Research Funds for the Central Universities (Grant Nos. 2412016KJ044 and 2412017FZ018 ). Appendix A
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/1
Y1 - 2018/1
N2 - Our previous paper reported the structure of ginseng pectic polysaccharides related to the cell migration inhibitory effects, but the underlying mechanisms are poorly understood. In this manuscript, rhamnogalacturonan I (RGI)-rich pectins prepared from ginseng pectin were investigated for their effect on cell migration. The results indicated that the combination of homogalacturonan (HG) and RGI-rich pectins exerted stronger effects than either HG- or RGI-rich pectin alone. Further studies revealed that the effects of HG- and RGI-rich pectins were dependent on pretreatment, which caused alterations in cell morphologies such as cell size and shape, focal adhesion, and the organization of actin filaments, suggesting that HG and RGI pectins exert synergistic effects on cell migration, likely through different ways. Morphological data and quantitative cell adhesion and spreading assays showed that HG- and RGI-rich pectin treatment decreased cell adhesion and cell spreading on the substratum, suggesting that HG- and RGI-rich pectins may exert their effects on cell migration via decreasing cell adhesion and cell spreading. Additionally, we showed that L-929 cells expressed little galectin-3 (Gal-3) and that lactose, an inhibitor of Gal-3 did not block the activities of HG- and RGI-rich pectins, implicating that cell migration inhibited by pectin did not correlate to Gal-3.
AB - Our previous paper reported the structure of ginseng pectic polysaccharides related to the cell migration inhibitory effects, but the underlying mechanisms are poorly understood. In this manuscript, rhamnogalacturonan I (RGI)-rich pectins prepared from ginseng pectin were investigated for their effect on cell migration. The results indicated that the combination of homogalacturonan (HG) and RGI-rich pectins exerted stronger effects than either HG- or RGI-rich pectin alone. Further studies revealed that the effects of HG- and RGI-rich pectins were dependent on pretreatment, which caused alterations in cell morphologies such as cell size and shape, focal adhesion, and the organization of actin filaments, suggesting that HG and RGI pectins exert synergistic effects on cell migration, likely through different ways. Morphological data and quantitative cell adhesion and spreading assays showed that HG- and RGI-rich pectin treatment decreased cell adhesion and cell spreading on the substratum, suggesting that HG- and RGI-rich pectins may exert their effects on cell migration via decreasing cell adhesion and cell spreading. Additionally, we showed that L-929 cells expressed little galectin-3 (Gal-3) and that lactose, an inhibitor of Gal-3 did not block the activities of HG- and RGI-rich pectins, implicating that cell migration inhibited by pectin did not correlate to Gal-3.
KW - Cell migration
KW - Galectin-3
KW - Ginseng pectin
KW - Homogalacturonan
KW - Rhamnogalacturonan I
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U2 - 10.1016/j.ijbiomac.2017.08.004
DO - 10.1016/j.ijbiomac.2017.08.004
M3 - Article
C2 - 28797814
AN - SCOPUS:85027420191
SN - 0141-8130
VL - 106
SP - 207
EP - 217
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -