The Rpd3 core complex is a chromatin stabilization module

Xiao Fen Chen, Benjamin Kuryan, Tasuku Kitada, Nancy Tran, Jing Yu Li, Siavash Kurdistani, Michael Grunstein, Bing Li, Michael Carey

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The S. cerevisiae Rpd3 large (Rpd3L) and small (Rpd3S) histone deacetylase (HDAC) complexes are prototypes for understanding transcriptional repression in eukaryotes [1]. The current view is that they function by deacetylating chromatin, thereby limiting accessibility of transcriptional factors to the underlying DNA. However, an Rpd3 catalytic mutant retains substantial repression capability when targeted to a promoter as a LexA fusion protein [2]. We investigated the HDAC-independent properties of the Rpd3 complexes biochemically and discovered a chaperone function, which promotes histone deposition onto DNA, and a novel activity, which prevents nucleosome eviction but not remodeling mediated by the ATP-dependent RSC complex. These HDAC-independent activities inhibit Pol II transcription on a nucleosomal template. The functions of the endogenous Rpd3 complexes can be recapitulated with recombinant Rpd3 core complex comprising Sin3, Rpd3, and Ume1. To test the hypothesis that Rpd3 contributes to chromatin stabilization in vivo, we measured histone H3 density genomewide and found that it was reduced at promoters in an Rpd3 deletion mutant but partially restored in a catalytic mutant. Importantly, the effects on H3 density are most apparent on RSC-enriched genes [3]. Our data suggest that the Rpd3 core complex could contribute to repression via a novel nucleosome stabilization function.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalCurrent Biology
Volume22
Issue number1
DOIs
StatePublished - Jan 10 2012

Fingerprint

histone deacetylase
Histone Deacetylases
Chromatin
chromatin
nucleosomes
Stabilization
Nucleosomes
histones
Histones
mutants
promoter regions
DNA
Transcription
Eukaryota
prototypes
Saccharomyces cerevisiae
eukaryotic cells
Fusion reactions
transcription factors
transcription (genetics)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chen, X. F., Kuryan, B., Kitada, T., Tran, N., Li, J. Y., Kurdistani, S., ... Carey, M. (2012). The Rpd3 core complex is a chromatin stabilization module. Current Biology, 22(1), 56-63. https://doi.org/10.1016/j.cub.2011.11.042

The Rpd3 core complex is a chromatin stabilization module. / Chen, Xiao Fen; Kuryan, Benjamin; Kitada, Tasuku; Tran, Nancy; Li, Jing Yu; Kurdistani, Siavash; Grunstein, Michael; Li, Bing; Carey, Michael.

In: Current Biology, Vol. 22, No. 1, 10.01.2012, p. 56-63.

Research output: Contribution to journalArticle

Chen, XF, Kuryan, B, Kitada, T, Tran, N, Li, JY, Kurdistani, S, Grunstein, M, Li, B & Carey, M 2012, 'The Rpd3 core complex is a chromatin stabilization module', Current Biology, vol. 22, no. 1, pp. 56-63. https://doi.org/10.1016/j.cub.2011.11.042
Chen XF, Kuryan B, Kitada T, Tran N, Li JY, Kurdistani S et al. The Rpd3 core complex is a chromatin stabilization module. Current Biology. 2012 Jan 10;22(1):56-63. https://doi.org/10.1016/j.cub.2011.11.042
Chen, Xiao Fen ; Kuryan, Benjamin ; Kitada, Tasuku ; Tran, Nancy ; Li, Jing Yu ; Kurdistani, Siavash ; Grunstein, Michael ; Li, Bing ; Carey, Michael. / The Rpd3 core complex is a chromatin stabilization module. In: Current Biology. 2012 ; Vol. 22, No. 1. pp. 56-63.
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