The Saccharomyces cerevisiae genome contains functional and nonfunctional copies of transposon Ty1.

J. D. Boeke, D. Eichinger, D. Castrillon, G. R. Fink

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

Saccharomyces cerevisiae Ty elements are transposons closely related to retroviruses. The DNA sequence of a functional Ty element (TyH3) is presented. The long terminal repeat sequences are different, suggesting that TyH3 is a recombinant Ty element. A chromosomal Ty element near the LYS2 gene, Ty173, was found to be nonfunctional, even though it has no detectable insertions or deletions. The defect in Ty173 transposition is caused by a missense mutation giving rise to a Leu-to-Ile substitution in the TYB (pol) open reading frame. Several chromosomal Ty elements carry this lesion in their DNA, indicating that nonfunctional Ty elements are common in the yeast genome.

Original languageEnglish (US)
Pages (from-to)1432-1442
Number of pages11
JournalMolecular and cellular biology
Volume8
Issue number4
DOIs
StatePublished - Apr 1988

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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