TY - JOUR
T1 - The Saccharomyces cerevisiae genome contains functional and nonfunctional copies of transposon Ty1.
AU - Boeke, J. D.
AU - Eichinger, D.
AU - Castrillon, D.
AU - Fink, G. R.
PY - 1988/4
Y1 - 1988/4
N2 - Saccharomyces cerevisiae Ty elements are transposons closely related to retroviruses. The DNA sequence of a functional Ty element (TyH3) is presented. The long terminal repeat sequences are different, suggesting that TyH3 is a recombinant Ty element. A chromosomal Ty element near the LYS2 gene, Ty173, was found to be nonfunctional, even though it has no detectable insertions or deletions. The defect in Ty173 transposition is caused by a missense mutation giving rise to a Leu-to-Ile substitution in the TYB (pol) open reading frame. Several chromosomal Ty elements carry this lesion in their DNA, indicating that nonfunctional Ty elements are common in the yeast genome.
AB - Saccharomyces cerevisiae Ty elements are transposons closely related to retroviruses. The DNA sequence of a functional Ty element (TyH3) is presented. The long terminal repeat sequences are different, suggesting that TyH3 is a recombinant Ty element. A chromosomal Ty element near the LYS2 gene, Ty173, was found to be nonfunctional, even though it has no detectable insertions or deletions. The defect in Ty173 transposition is caused by a missense mutation giving rise to a Leu-to-Ile substitution in the TYB (pol) open reading frame. Several chromosomal Ty elements carry this lesion in their DNA, indicating that nonfunctional Ty elements are common in the yeast genome.
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U2 - 10.1128/MCB.8.4.1432
DO - 10.1128/MCB.8.4.1432
M3 - Article
C2 - 2837641
AN - SCOPUS:0023990722
SN - 0270-7306
VL - 8
SP - 1432
EP - 1442
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 4
ER -