The splice variants of D-AKAP1 differ in subcellular localization and tissue-specific expression

Lily Jun Shen Huang, Kyle Durick, Susan S. Taylor

Research output: Contribution to journalArticle

Abstract

Unlike other known A-Kinase anchoring proteins (AKAPs) which tether the type II regulatory subunit of cAMP-dependent protein kinase. I)-AKAP1 can bind both the type I and the type II regulatory subunit, cl)NA cloning of I)-AKAPI revealed that in addition to the 526 amino acid core. there are two N-terminal splices, and two C-terminal splice. To determine the expression pattern of these different splice variants, a multiple tissue Northern blot was probed with splice-specific cDNA probes. The NI splice was specifically expressed in liver, and the C1 splice was predominantly expressed in testis. The C2 splice had a wide tissue distribution similar to the overall expression pab tern. "Using microinjection techniques, the NO splice of D-AKAP1 was found to target to the mitochondria, while the N1 splice primarily localized to the endoptasmic reticulum. The co-expression of the D-AKAPla isoform, which employs the NO splice, and RIIb in mitochondria-rich brown adipose tissues and their interaction in vitro indicated that such interaction could be physiologically important.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - 1997

Fingerprint

Mitochondria
mitochondria
Tissue
Charadriiformes
Reticulum
Brown Adipose Tissue
brown adipose tissue
cAMP-dependent protein kinase
reticulum
Microinjections
tissue distribution
Tissue Distribution
Cyclic AMP-Dependent Protein Kinases
Laridae
Northern blotting
Northern Blotting
protein kinases
Protein Kinases
Testis
Organism Cloning

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

The splice variants of D-AKAP1 differ in subcellular localization and tissue-specific expression. / Huang, Lily Jun Shen; Durick, Kyle; Taylor, Susan S.

In: FASEB Journal, Vol. 11, No. 9, 1997.

Research output: Contribution to journalArticle

@article{605d6a59e0a043f18add8b27c4959e09,
title = "The splice variants of D-AKAP1 differ in subcellular localization and tissue-specific expression",
abstract = "Unlike other known A-Kinase anchoring proteins (AKAPs) which tether the type II regulatory subunit of cAMP-dependent protein kinase. I)-AKAP1 can bind both the type I and the type II regulatory subunit, cl)NA cloning of I)-AKAPI revealed that in addition to the 526 amino acid core. there are two N-terminal splices, and two C-terminal splice. To determine the expression pattern of these different splice variants, a multiple tissue Northern blot was probed with splice-specific cDNA probes. The NI splice was specifically expressed in liver, and the C1 splice was predominantly expressed in testis. The C2 splice had a wide tissue distribution similar to the overall expression pab tern. {"}Using microinjection techniques, the NO splice of D-AKAP1 was found to target to the mitochondria, while the N1 splice primarily localized to the endoptasmic reticulum. The co-expression of the D-AKAPla isoform, which employs the NO splice, and RIIb in mitochondria-rich brown adipose tissues and their interaction in vitro indicated that such interaction could be physiologically important.",
author = "Huang, {Lily Jun Shen} and Kyle Durick and Taylor, {Susan S.}",
year = "1997",
language = "English (US)",
volume = "11",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "9",

}

TY - JOUR

T1 - The splice variants of D-AKAP1 differ in subcellular localization and tissue-specific expression

AU - Huang, Lily Jun Shen

AU - Durick, Kyle

AU - Taylor, Susan S.

PY - 1997

Y1 - 1997

N2 - Unlike other known A-Kinase anchoring proteins (AKAPs) which tether the type II regulatory subunit of cAMP-dependent protein kinase. I)-AKAP1 can bind both the type I and the type II regulatory subunit, cl)NA cloning of I)-AKAPI revealed that in addition to the 526 amino acid core. there are two N-terminal splices, and two C-terminal splice. To determine the expression pattern of these different splice variants, a multiple tissue Northern blot was probed with splice-specific cDNA probes. The NI splice was specifically expressed in liver, and the C1 splice was predominantly expressed in testis. The C2 splice had a wide tissue distribution similar to the overall expression pab tern. "Using microinjection techniques, the NO splice of D-AKAP1 was found to target to the mitochondria, while the N1 splice primarily localized to the endoptasmic reticulum. The co-expression of the D-AKAPla isoform, which employs the NO splice, and RIIb in mitochondria-rich brown adipose tissues and their interaction in vitro indicated that such interaction could be physiologically important.

AB - Unlike other known A-Kinase anchoring proteins (AKAPs) which tether the type II regulatory subunit of cAMP-dependent protein kinase. I)-AKAP1 can bind both the type I and the type II regulatory subunit, cl)NA cloning of I)-AKAPI revealed that in addition to the 526 amino acid core. there are two N-terminal splices, and two C-terminal splice. To determine the expression pattern of these different splice variants, a multiple tissue Northern blot was probed with splice-specific cDNA probes. The NI splice was specifically expressed in liver, and the C1 splice was predominantly expressed in testis. The C2 splice had a wide tissue distribution similar to the overall expression pab tern. "Using microinjection techniques, the NO splice of D-AKAP1 was found to target to the mitochondria, while the N1 splice primarily localized to the endoptasmic reticulum. The co-expression of the D-AKAPla isoform, which employs the NO splice, and RIIb in mitochondria-rich brown adipose tissues and their interaction in vitro indicated that such interaction could be physiologically important.

UR - http://www.scopus.com/inward/record.url?scp=33750171022&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750171022&partnerID=8YFLogxK

M3 - Article

VL - 11

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 9

ER -