The structure of bacterial DnaA: Implications for general mechanisms underlying DNA replication initiation

Jan P. Erzberger, Michelle M. Pirruccello, James M. Berger

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

The initiation of DNA replication is a key event in the cell cycle of all organisms. In bacteria, replication initiation occurs at specific origin sequences that are recognized and processed by an oligomeric complex of the initiator protein DnaA. We have determined the structure of the conserved core of the Aquifex aeolicus DnaA protein to 2.7 Å resolution. The protein comprises an AAA+ nucleotide-binding fold linked through a long, helical connector to an all-helical DNA-binding domain. The structure serves as a template for understanding the physical consequences of a variety of DnaA mutations, and conserved motifs in the protein suggest how two critical aspects of origin processing, DNA binding and homo-oligomerization, are mediated. The spatial arrangement of these motifs in DnaA is similar to that of the eukaryotic-like archaeal replication initiation factor Cdc6/Orc1, demonstrating that mechanistic elements of origin processing may be conserved across bacterial, archaeal and eukaryotic domains of life.

Original languageEnglish (US)
Pages (from-to)4763-4773
Number of pages11
JournalEMBO Journal
Volume21
Issue number18
DOIs
StatePublished - Sep 16 2002

Fingerprint

Bacterial Structures
DNA Replication
DNA
Peptide Initiation Factors
Amino Acid Motifs
Proteins
Oligomerization
Cell Cycle
Nucleotides
Processing
Bacteria
Mutation
Cells

Keywords

  • Bacteria
  • DNA replication initiation
  • DnaA
  • Structure

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

The structure of bacterial DnaA : Implications for general mechanisms underlying DNA replication initiation. / Erzberger, Jan P.; Pirruccello, Michelle M.; Berger, James M.

In: EMBO Journal, Vol. 21, No. 18, 16.09.2002, p. 4763-4773.

Research output: Contribution to journalArticle

Erzberger, Jan P. ; Pirruccello, Michelle M. ; Berger, James M. / The structure of bacterial DnaA : Implications for general mechanisms underlying DNA replication initiation. In: EMBO Journal. 2002 ; Vol. 21, No. 18. pp. 4763-4773.
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