The structure of bacterial DnaA: Implications for general mechanisms underlying DNA replication initiation

Jan P. Erzberger, Michelle M. Pirruccello, James M. Berger

Research output: Contribution to journalArticle

181 Scopus citations

Abstract

The initiation of DNA replication is a key event in the cell cycle of all organisms. In bacteria, replication initiation occurs at specific origin sequences that are recognized and processed by an oligomeric complex of the initiator protein DnaA. We have determined the structure of the conserved core of the Aquifex aeolicus DnaA protein to 2.7 Å resolution. The protein comprises an AAA+ nucleotide-binding fold linked through a long, helical connector to an all-helical DNA-binding domain. The structure serves as a template for understanding the physical consequences of a variety of DnaA mutations, and conserved motifs in the protein suggest how two critical aspects of origin processing, DNA binding and homo-oligomerization, are mediated. The spatial arrangement of these motifs in DnaA is similar to that of the eukaryotic-like archaeal replication initiation factor Cdc6/Orc1, demonstrating that mechanistic elements of origin processing may be conserved across bacterial, archaeal and eukaryotic domains of life.

Original languageEnglish (US)
Pages (from-to)4763-4773
Number of pages11
JournalEMBO Journal
Volume21
Issue number18
DOIs
StatePublished - Sep 16 2002

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Keywords

  • Bacteria
  • DNA replication initiation
  • DnaA
  • Structure

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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