The structure of spinach Photosystem I studied by electron microscopy

Egbert J. Boekema; R. Max Wynn; Richard Malkin

doi:10.1016/0005-2728(90)90177-6

The structure of spinach Photosystem I studied by electron microscopy

Egbert J. Boekema, R. Max Wynn, Richard Malkin

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.

Original language	English (US)
Pages (from-to)	49-56
Number of pages	8
Journal	BBA - Bioenergetics
Volume	1017
Issue number	1
DOIs	https://doi.org/10.1016/0005-2728(90)90177-6
State	Published - May 15 1990

Keywords

(Spinach)
Electron microscopy
Image analysis
Photosystem I structure

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(90)90177-6

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title = "The structure of spinach Photosystem I studied by electron microscopy",

abstract = "The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.",

keywords = "(Spinach), Electron microscopy, Image analysis, Photosystem I structure",

author = "Boekema, {Egbert J.} and Wynn, {R. Max} and Richard Malkin",

note = "Funding Information: We wish to thank Prof. E.F.J. van Bruggen for general support, Dr. W. Keegstra for his help with computer image analysis, Mr. J. Haker for technical assistance and Mr. K. Gilissen for photography. This work was supported by grants from the Royal Netherlands Academy of Sciences (KNAW) to E.J.B. and the National Science Foundation to R.M.",

year = "1990",

month = may,

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doi = "10.1016/0005-2728(90)90177-6",

language = "English (US)",

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AU - Boekema, Egbert J.

AU - Wynn, R. Max

AU - Malkin, Richard

N1 - Funding Information: We wish to thank Prof. E.F.J. van Bruggen for general support, Dr. W. Keegstra for his help with computer image analysis, Mr. J. Haker for technical assistance and Mr. K. Gilissen for photography. This work was supported by grants from the Royal Netherlands Academy of Sciences (KNAW) to E.J.B. and the National Science Foundation to R.M.

PY - 1990/5/15

Y1 - 1990/5/15

N2 - The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.

AB - The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.

KW - (Spinach)

KW - Electron microscopy

KW - Image analysis

KW - Photosystem I structure

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The structure of spinach Photosystem I studied by electron microscopy

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