The structure of spinach Photosystem I studied by electron microscopy

Egbert J. Boekema, R. Max Wynn, Richard Malkin

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.

Original languageEnglish (US)
Pages (from-to)49-56
Number of pages8
JournalBBA - Bioenergetics
Volume1017
Issue number1
DOIs
StatePublished - May 15 1990

Keywords

  • (Spinach)
  • Electron microscopy
  • Image analysis
  • Photosystem I structure

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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