The structure of spinach Photosystem I studied by electron microscopy

Egbert J. Boekema, R. Max Wynn, Richard Malkin

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and side views) of a native PS I complex (PSI-200), an antenna-depleted PS I complex (PSI-100) and the PS I reaction center complex (CPI) were analyzed. The overall structure of the native PS I complex was found to be a disk with dimensions (corrected for attached detergent) of 16 × 12 nm in the plane of the membrane and a height of 6.8 nm. The PSI-100 and CPI complexes gave much smaller projections but these were similar in shape and size to those of the previously analyzed cyanobacterial PS I complex. The arrangement of the subunits within the native PS I complex is discussed. It is concluded that a shell of about eight light-harvesting complex (LHCI) subunits attached to the PSI-100 complex fits the dimensions and shape of the PSI-200 complex.

Original languageEnglish (US)
Pages (from-to)49-56
Number of pages8
JournalBBA - Bioenergetics
Volume1017
Issue number1
DOIs
StatePublished - May 15 1990

Fingerprint

Photosystem I Protein Complex
Spinacia oleracea
Electron microscopy
Electron Microscopy
Chloroplasts
Detergents
Image analysis
Antennas
Membranes
Light

Keywords

  • (Spinach)
  • Electron microscopy
  • Image analysis
  • Photosystem I structure

ASJC Scopus subject areas

  • Biophysics

Cite this

The structure of spinach Photosystem I studied by electron microscopy. / Boekema, Egbert J.; Wynn, R. Max; Malkin, Richard.

In: BBA - Bioenergetics, Vol. 1017, No. 1, 15.05.1990, p. 49-56.

Research output: Contribution to journalArticle

Boekema, Egbert J. ; Wynn, R. Max ; Malkin, Richard. / The structure of spinach Photosystem I studied by electron microscopy. In: BBA - Bioenergetics. 1990 ; Vol. 1017, No. 1. pp. 49-56.
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