The structure of the first representative of Pfam family PF09836 reveals a two-domain organization and suggests involvement in transcriptional regulation

Debanu Das, Nick V. Grishin, Abhinav Kumar, Dennis Carlton, Constantina Bakolitsa, Mitchell D. Miller, Polat Abdubek, Tamara Astakhova, Herbert L. Axelrod, Prasad Burra, Connie Chen, Hsiu Ju Chiu, Michelle Chiu, Thomas Clayton, Marc C. Deller, Lian Duan, Kyle Ellrott, Dustin Ernst, Carol L. Farr, Julie FeuerhelmAnna Grzechnik, Slawomir K. Grzechnik, Joanna C. Grant, Gye Won Han, Lukasz Jaroszewski, Kevin K. Jin, Hope A. Johnson, Heath E. Klock, Mark W. Knuth, Piotr Kozbial, S. Sri Krishna, David Marciano, Daniel McMullan, Andrew T. Morse, Edward Nigoghossian, Amanda Nopakun, Linda Okach, Silvya Oommachen, Jessica Paulsen, Christina Puckett, Ron Reyes, Christopher L. Rife, Natasha Sefcovic, Henry J. Tien, Christine B. Trame, Henry Van Den Bedem, Dana Weekes, Tiffany Wooten, Qingping Xu, Keith O. Hodgson, John Wooley, Marc André Elsliger, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Ian A. Wilson

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Proteins with the DUF2063 domain constitute a new Pfam family, PF09836. The crystal structure of a member of this family, NGO1945 from Neisseria gonorrhoeae, has been determined and reveals that the N-terminal DUF2063 domain is likely to be a DNA-binding domain. In conjunction with the rest of the protein, NGO1945 is likely to be involved in transcriptional regulation, which is consistent with genomic neighborhood analysis. Of the 216 currently known proteins that contain a DUF2063 domain, the most significant sequence homologs of NGO1945 (̃40-99% sequence identity) are from various Neisseria and Haemophilus species. As these are important human pathogens, NGO1945 represents an interesting candidate for further exploration via biochemical studies and possible therapeutic intervention.

Original languageEnglish (US)
Pages (from-to)1174-1181
Number of pages8
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number10
DOIs
StatePublished - Oct 1 2010

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Keywords

  • DUF2063
  • NGO1945
  • PF09836
  • putative DNA-binding proteins
  • putative transcription regulators
  • structural genomics

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Das, D., Grishin, N. V., Kumar, A., Carlton, D., Bakolitsa, C., Miller, M. D., Abdubek, P., Astakhova, T., Axelrod, H. L., Burra, P., Chen, C., Chiu, H. J., Chiu, M., Clayton, T., Deller, M. C., Duan, L., Ellrott, K., Ernst, D., Farr, C. L., ... Wilson, I. A. (2010). The structure of the first representative of Pfam family PF09836 reveals a two-domain organization and suggests involvement in transcriptional regulation. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 66(10), 1174-1181. https://doi.org/10.1107/S1744309109022672