The structure of the G protein heterotrimer Giα1β1γ2

Mark A. Wall, David E. Coleman, Ethan Lee, Jorge A. Iñiguez-Lluhi, Bruce A. Posner, Alfred G. Gilman, Stephen R. Sprang

Research output: Contribution to journalArticle

960 Citations (Scopus)

Abstract

The crystallographic structure of the G protein heterotrimer Giα1(GDP)β1γ2 (at 2.3 A) reveals two nonoverlapping regions of contact between α and β, an extended interface between β and nearly all of γ, and limited interaction of α with γ. The major α/β interface covers switch II of α, and GTP-induced rearrangement of switch II causes subunit dissociation during signaling. Alterations in GDP binding in the heterotrimer (compared with α-GDP) explain stabilization of the inactive conformation of α by βγ. Repeated WD motifs in β form a circularized sevenfold β propeller. The conserved cores of these motifs are a scaffold for display of their more variable linkers on the exterior face of each propeller blade.

Original languageEnglish (US)
Pages (from-to)1047-1058
Number of pages12
JournalCell
Volume83
Issue number6
DOIs
StatePublished - Dec 15 1995

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Propellers
Guanosine Triphosphate
GTP-Binding Proteins
Switches
Scaffolds
Conformations
Stabilization
Display devices

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Molecular Biology

Cite this

Wall, M. A., Coleman, D. E., Lee, E., Iñiguez-Lluhi, J. A., Posner, B. A., Gilman, A. G., & Sprang, S. R. (1995). The structure of the G protein heterotrimer Giα1β1γ2. Cell, 83(6), 1047-1058. https://doi.org/10.1016/0092-8674(95)90220-1

The structure of the G protein heterotrimer Giα1β1γ2. / Wall, Mark A.; Coleman, David E.; Lee, Ethan; Iñiguez-Lluhi, Jorge A.; Posner, Bruce A.; Gilman, Alfred G.; Sprang, Stephen R.

In: Cell, Vol. 83, No. 6, 15.12.1995, p. 1047-1058.

Research output: Contribution to journalArticle

Wall, MA, Coleman, DE, Lee, E, Iñiguez-Lluhi, JA, Posner, BA, Gilman, AG & Sprang, SR 1995, 'The structure of the G protein heterotrimer Giα1β1γ2', Cell, vol. 83, no. 6, pp. 1047-1058. https://doi.org/10.1016/0092-8674(95)90220-1
Wall MA, Coleman DE, Lee E, Iñiguez-Lluhi JA, Posner BA, Gilman AG et al. The structure of the G protein heterotrimer Giα1β1γ2. Cell. 1995 Dec 15;83(6):1047-1058. https://doi.org/10.1016/0092-8674(95)90220-1
Wall, Mark A. ; Coleman, David E. ; Lee, Ethan ; Iñiguez-Lluhi, Jorge A. ; Posner, Bruce A. ; Gilman, Alfred G. ; Sprang, Stephen R. / The structure of the G protein heterotrimer Giα1β1γ2. In: Cell. 1995 ; Vol. 83, No. 6. pp. 1047-1058.
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