The study of biogenesis and secretion of alkaline phosphatase and its mutant forms in Escherichia coli. I. Introduction of mutations into alkaline phosphatase gene

A. L. Karamyshev, M. G. Shlyapnikov, M. I. Khmelnitsky, M. A. Nesmeyanova, V. N. Ksenzenko

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Abstract

Various mutations in E. coli alkaline phosphatase gene were obtained by oligonucleotide-directed mutagenesis. They result in amino acid substitutions in the signal peptide cleavage site [Val for Ala(-1)] and in the N terminus of mature polypeptide chain: Ala for Arg(+1) and Gln for Glu(+4); Gln for Glu(+4). Enzyme activity was observed in all E. coli strains transformed by plasmids with cloned mutant genes. In addition, an amber mutation was introduced into the Arg(+1) position, and the synthesis of mutant alkaline phosphatase was shown in E. coli strains containing suppressor tRNAs specific for Ser, Gln, Tyr, Leu, Ala, Glu, Phe, Gly, His, Pro, and Cys.

Original languageEnglish (US)
Pages (from-to)150-157
Number of pages8
JournalMolekulyarnaya Biologiya
Volume28
Issue number1
StatePublished - 1994

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Alkaline Phosphatase
Escherichia coli
Mutation
RNA, Transfer, Ser
Genes
Amino Acid Substitution
Protein Sorting Signals
Site-Directed Mutagenesis
Plasmids
Peptides
Enzymes

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Karamyshev, A. L., Shlyapnikov, M. G., Khmelnitsky, M. I., Nesmeyanova, M. A., & Ksenzenko, V. N. (1994). The study of biogenesis and secretion of alkaline phosphatase and its mutant forms in Escherichia coli. I. Introduction of mutations into alkaline phosphatase gene. Molekulyarnaya Biologiya, 28(1), 150-157.

The study of biogenesis and secretion of alkaline phosphatase and its mutant forms in Escherichia coli. I. Introduction of mutations into alkaline phosphatase gene. / Karamyshev, A. L.; Shlyapnikov, M. G.; Khmelnitsky, M. I.; Nesmeyanova, M. A.; Ksenzenko, V. N.

In: Molekulyarnaya Biologiya, Vol. 28, No. 1, 1994, p. 150-157.

Research output: Contribution to journalArticle

Karamyshev, A. L. ; Shlyapnikov, M. G. ; Khmelnitsky, M. I. ; Nesmeyanova, M. A. ; Ksenzenko, V. N. / The study of biogenesis and secretion of alkaline phosphatase and its mutant forms in Escherichia coli. I. Introduction of mutations into alkaline phosphatase gene. In: Molekulyarnaya Biologiya. 1994 ; Vol. 28, No. 1. pp. 150-157.
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