The swelling-activated chloride channel CIC-2, the chloride channel CIC- 3, and CIC-5, a chloride channel mutated in kidney stone disease, are expressed in distinct subpopulations of renal epithelial cells

Nicholas Obermüller, Norbert Gretz, Wilhelm Kriz, Robert F. Reilly, Ralph Witzgall

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The mammalian genome encodes at least nine different members of the CIC family of chloride channels. So far only two of them could be localized on a cellular level in the kidney. We now report on the precise intrarenal localization of the mRNAs coding for the chloride channels CIC-2, CIC-3 and CIC-5. Expression of CIC-2 mRNA, encoding a swelling-activated chloride channel, could be demonstrated in the S3 segment of the proximal tubule. The chloride channel CIC-3 mRNA and CIC-5 mRNA, coding for a chloride channel mutated in kidney stone disease, were both expressed in intercalated cells of the connecting tubule and collecting duct. Whereas CIC-3 mRNA expression was most prominent in the cortex of rat kidneys, CIC-5 mRNA was expressed from the cortex through the upper portion of the inner medulla. A detailed analysis revealed that CIC-3 was expressed by type B intercalated cells, whereas CIC-5 was expressed by type A intercalated cells. These findings have important implications for the pathogenesis of hereditary kidney stone disease caused by mutations in the CLCN5 gene.

Original languageEnglish (US)
Pages (from-to)635-642
Number of pages8
JournalJournal of Clinical Investigation
Volume101
Issue number3
StatePublished - Feb 1 1998

Fingerprint

Chloride Channels
Kidney Calculi
Kidney Diseases
Epithelial Cells
Kidney
Messenger RNA
Kidney Cortex
ClC-3 channel
CLC-5 chloride channel
Genome
Mutation
Genes

Keywords

  • Chloride channels
  • Genetic disease
  • Intercalated cells
  • Kidney
  • Nephrolithiasis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The swelling-activated chloride channel CIC-2, the chloride channel CIC- 3, and CIC-5, a chloride channel mutated in kidney stone disease, are expressed in distinct subpopulations of renal epithelial cells. / Obermüller, Nicholas; Gretz, Norbert; Kriz, Wilhelm; Reilly, Robert F.; Witzgall, Ralph.

In: Journal of Clinical Investigation, Vol. 101, No. 3, 01.02.1998, p. 635-642.

Research output: Contribution to journalArticle

@article{e4fcc44919284302bec6397a978fedf9,
title = "The swelling-activated chloride channel CIC-2, the chloride channel CIC- 3, and CIC-5, a chloride channel mutated in kidney stone disease, are expressed in distinct subpopulations of renal epithelial cells",
abstract = "The mammalian genome encodes at least nine different members of the CIC family of chloride channels. So far only two of them could be localized on a cellular level in the kidney. We now report on the precise intrarenal localization of the mRNAs coding for the chloride channels CIC-2, CIC-3 and CIC-5. Expression of CIC-2 mRNA, encoding a swelling-activated chloride channel, could be demonstrated in the S3 segment of the proximal tubule. The chloride channel CIC-3 mRNA and CIC-5 mRNA, coding for a chloride channel mutated in kidney stone disease, were both expressed in intercalated cells of the connecting tubule and collecting duct. Whereas CIC-3 mRNA expression was most prominent in the cortex of rat kidneys, CIC-5 mRNA was expressed from the cortex through the upper portion of the inner medulla. A detailed analysis revealed that CIC-3 was expressed by type B intercalated cells, whereas CIC-5 was expressed by type A intercalated cells. These findings have important implications for the pathogenesis of hereditary kidney stone disease caused by mutations in the CLCN5 gene.",
keywords = "Chloride channels, Genetic disease, Intercalated cells, Kidney, Nephrolithiasis",
author = "Nicholas Oberm{\"u}ller and Norbert Gretz and Wilhelm Kriz and Reilly, {Robert F.} and Ralph Witzgall",
year = "1998",
month = "2",
day = "1",
language = "English (US)",
volume = "101",
pages = "635--642",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "3",

}

TY - JOUR

T1 - The swelling-activated chloride channel CIC-2, the chloride channel CIC- 3, and CIC-5, a chloride channel mutated in kidney stone disease, are expressed in distinct subpopulations of renal epithelial cells

AU - Obermüller, Nicholas

AU - Gretz, Norbert

AU - Kriz, Wilhelm

AU - Reilly, Robert F.

AU - Witzgall, Ralph

PY - 1998/2/1

Y1 - 1998/2/1

N2 - The mammalian genome encodes at least nine different members of the CIC family of chloride channels. So far only two of them could be localized on a cellular level in the kidney. We now report on the precise intrarenal localization of the mRNAs coding for the chloride channels CIC-2, CIC-3 and CIC-5. Expression of CIC-2 mRNA, encoding a swelling-activated chloride channel, could be demonstrated in the S3 segment of the proximal tubule. The chloride channel CIC-3 mRNA and CIC-5 mRNA, coding for a chloride channel mutated in kidney stone disease, were both expressed in intercalated cells of the connecting tubule and collecting duct. Whereas CIC-3 mRNA expression was most prominent in the cortex of rat kidneys, CIC-5 mRNA was expressed from the cortex through the upper portion of the inner medulla. A detailed analysis revealed that CIC-3 was expressed by type B intercalated cells, whereas CIC-5 was expressed by type A intercalated cells. These findings have important implications for the pathogenesis of hereditary kidney stone disease caused by mutations in the CLCN5 gene.

AB - The mammalian genome encodes at least nine different members of the CIC family of chloride channels. So far only two of them could be localized on a cellular level in the kidney. We now report on the precise intrarenal localization of the mRNAs coding for the chloride channels CIC-2, CIC-3 and CIC-5. Expression of CIC-2 mRNA, encoding a swelling-activated chloride channel, could be demonstrated in the S3 segment of the proximal tubule. The chloride channel CIC-3 mRNA and CIC-5 mRNA, coding for a chloride channel mutated in kidney stone disease, were both expressed in intercalated cells of the connecting tubule and collecting duct. Whereas CIC-3 mRNA expression was most prominent in the cortex of rat kidneys, CIC-5 mRNA was expressed from the cortex through the upper portion of the inner medulla. A detailed analysis revealed that CIC-3 was expressed by type B intercalated cells, whereas CIC-5 was expressed by type A intercalated cells. These findings have important implications for the pathogenesis of hereditary kidney stone disease caused by mutations in the CLCN5 gene.

KW - Chloride channels

KW - Genetic disease

KW - Intercalated cells

KW - Kidney

KW - Nephrolithiasis

UR - http://www.scopus.com/inward/record.url?scp=2642687664&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2642687664&partnerID=8YFLogxK

M3 - Article

VL - 101

SP - 635

EP - 642

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 3

ER -