The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity

Michal Wieczorek, Joseph Tcherkezian, Cynthia Bernier, Andrea E. Prota, Sami Chaaban, Yannève Rolland, Claude Godbout, Mark A. Hancock, Joseph C. Arezzo, Ozhan Ocal, Cecilia Rocha, Natacha Olieric, Anita Hall, Hui Ding, Alexandre Bramoullé, Matthew G. Annis, George Zogopoulos, Patrick G. Harran, Thomas M. Wilkie, Rolf A. Brekken & 5 others Peter M. Siegel, Michel O. Steinmetz, Gordon C. Shore, Gary J. Brouhard, Anne Roulston

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Microtubule-targeting agents (MTAs) are widely used anticancer agents, but toxicities such as neuropathy limit their clinical use. MTAs bind to and alter the stability of microtubules, causing cell death in mitosis. We describe DZ-2384, a preclinical compound that exhibits potent antitumor activity in models of multiple cancer types. It has an unusually high safety margin and lacks neurotoxicity in rats at effective plasma concentrations. DZ-2384 binds the vinca domain of tubulin in a distinct way, imparting structurally and functionally different effects on microtubule dynamics compared to other vinca-binding compounds. X-ray crystallography and electron microscopy studies demonstrate that DZ-2384 causes straightening of curved protofilaments, an effect proposed to favor polymerization of tubulin. Both DZ-2384 and the vinca alkaloid vinorelbine inhibit microtubule growth rate; however, DZ-2384 increases the rescue frequency and preserves the microtubule network in nonmitotic cells and in primary neurons. This differential modulation of tubulin results in a potent MTA therapeutic with enhanced safety.

Original languageEnglish (US)
JournalScience Translational Medicine
Volume8
Issue number365
DOIs
StatePublished - Nov 16 2016

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Microtubules
Tubulin
Vinca
Vinca Alkaloids
Safety
X Ray Crystallography
DZ-2384
Mitosis
Polymerization
Antineoplastic Agents
Electron Microscopy
Cell Death
Neurons
Growth
Neoplasms

ASJC Scopus subject areas

  • Medicine(all)

Cite this

The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity. / Wieczorek, Michal; Tcherkezian, Joseph; Bernier, Cynthia; Prota, Andrea E.; Chaaban, Sami; Rolland, Yannève; Godbout, Claude; Hancock, Mark A.; Arezzo, Joseph C.; Ocal, Ozhan; Rocha, Cecilia; Olieric, Natacha; Hall, Anita; Ding, Hui; Bramoullé, Alexandre; Annis, Matthew G.; Zogopoulos, George; Harran, Patrick G.; Wilkie, Thomas M.; Brekken, Rolf A.; Siegel, Peter M.; Steinmetz, Michel O.; Shore, Gordon C.; Brouhard, Gary J.; Roulston, Anne.

In: Science Translational Medicine, Vol. 8, No. 365, 16.11.2016.

Research output: Contribution to journalArticle

Wieczorek, M, Tcherkezian, J, Bernier, C, Prota, AE, Chaaban, S, Rolland, Y, Godbout, C, Hancock, MA, Arezzo, JC, Ocal, O, Rocha, C, Olieric, N, Hall, A, Ding, H, Bramoullé, A, Annis, MG, Zogopoulos, G, Harran, PG, Wilkie, TM, Brekken, RA, Siegel, PM, Steinmetz, MO, Shore, GC, Brouhard, GJ & Roulston, A 2016, 'The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity', Science Translational Medicine, vol. 8, no. 365. https://doi.org/10.1126/scitranslmed.aag1093
Wieczorek, Michal ; Tcherkezian, Joseph ; Bernier, Cynthia ; Prota, Andrea E. ; Chaaban, Sami ; Rolland, Yannève ; Godbout, Claude ; Hancock, Mark A. ; Arezzo, Joseph C. ; Ocal, Ozhan ; Rocha, Cecilia ; Olieric, Natacha ; Hall, Anita ; Ding, Hui ; Bramoullé, Alexandre ; Annis, Matthew G. ; Zogopoulos, George ; Harran, Patrick G. ; Wilkie, Thomas M. ; Brekken, Rolf A. ; Siegel, Peter M. ; Steinmetz, Michel O. ; Shore, Gordon C. ; Brouhard, Gary J. ; Roulston, Anne. / The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity. In: Science Translational Medicine. 2016 ; Vol. 8, No. 365.
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