The TBN protein, which is essential for early embryonic mouse development, is an inducible TAFII implicated in adipogenesis

Mohamed Guermah, Kai Ge, Cheng Ming Chiang, Robert G. Roeder

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Human TFIID contains the TATA-binding protein (TBP) and several TBP-associated factors (hTAFs) that have been shown to play important roles, within TFIID, both in core promoter recognition and as coactivators. Here we show that the human TAFII43 (TAF8) is an integral component of a functional TFIID and an apparent ortholog to the recently reported mouse TBN, which is essential for early embryonic mouse developmental events. Significantly, we also show that TAF8 is dramatically induced and sequestered within TFIID upon differentiation of 3T3-L1 preadipocytes to adipocytes, whereas the expression of all other TAFs tested is slightly reduced. Moreover, when ectopically expressed, the histone fold domain of TAF8 acts as a dominant-negative mutant and selectively inhibits 3T3-L1 adipogenic differentiation. Furthermore TAF8 acts as a positive regulator of adipogenesis and reverses the inhibitory effect of its histone fold. These data suggest a selective role for TAF8 in a specific cell differentiation process(es).

Original languageEnglish (US)
Pages (from-to)991-1001
Number of pages11
JournalMolecular Cell
Volume12
Issue number4
DOIs
Publication statusPublished - Oct 2003

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ASJC Scopus subject areas

  • Molecular Biology

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