The TEAD/TEF family protein Scalloped mediates transcriptional output of the Hippo growth-regulatory pathway.

Shian Wu, Y. Liu, Yonggang Zheng, Jixin Dong, Duojia Pan

Research output: Contribution to journalArticle

385 Citations (Scopus)

Abstract

The Hippo (Hpo) kinase cascade restricts tissue growth by inactivating the transcriptional coactivator Yorkie (Yki), which regulates the expression of target genes such as the cell death inhibitor diap1 by unknown mechanisms. Here we identify the TEAD/TEF family protein Scalloped (Sd) as a DNA-binding transcription factor that partners with Yki to mediate the transcriptional output of the Hpo growth-regulatory pathway. The diap1 (th) locus harbors a minimal Sd-binding Hpo Responsive Element (HRE) that mediates transcriptional regulation by the Hpo pathway. Sd binds directly to Yki, and a Yki missense mutation that abrogates Sd-Yki binding also inactivates Yki function in vivo. We further demonstrate that sd is required for yki-induced tissue overgrowth and target gene expression, and that sd activity is conserved in its mammalian homolog. Our results uncover a heretofore missing link in the Hpo signaling pathway and provide a glimpse of the molecular events on a Hpo-responsive enhancer element.

Original languageEnglish (US)
Pages (from-to)388-398
Number of pages11
JournalDevelopmental Cell
Volume14
Issue number3
DOIs
StatePublished - Jan 1 2008

Fingerprint

Tissue
Gene Expression
Cell death
Missense Mutation
Growth
Ports and harbors
Gene expression
Proteins
Cell Death
Transcription Factors
Phosphotransferases
Genes
DNA

ASJC Scopus subject areas

  • Developmental Biology

Cite this

The TEAD/TEF family protein Scalloped mediates transcriptional output of the Hippo growth-regulatory pathway. / Wu, Shian; Liu, Y.; Zheng, Yonggang; Dong, Jixin; Pan, Duojia.

In: Developmental Cell, Vol. 14, No. 3, 01.01.2008, p. 388-398.

Research output: Contribution to journalArticle

Wu, Shian ; Liu, Y. ; Zheng, Yonggang ; Dong, Jixin ; Pan, Duojia. / The TEAD/TEF family protein Scalloped mediates transcriptional output of the Hippo growth-regulatory pathway. In: Developmental Cell. 2008 ; Vol. 14, No. 3. pp. 388-398.
@article{00e5de694fb94d3a924e3ecb1a7d37ea,
title = "The TEAD/TEF family protein Scalloped mediates transcriptional output of the Hippo growth-regulatory pathway.",
abstract = "The Hippo (Hpo) kinase cascade restricts tissue growth by inactivating the transcriptional coactivator Yorkie (Yki), which regulates the expression of target genes such as the cell death inhibitor diap1 by unknown mechanisms. Here we identify the TEAD/TEF family protein Scalloped (Sd) as a DNA-binding transcription factor that partners with Yki to mediate the transcriptional output of the Hpo growth-regulatory pathway. The diap1 (th) locus harbors a minimal Sd-binding Hpo Responsive Element (HRE) that mediates transcriptional regulation by the Hpo pathway. Sd binds directly to Yki, and a Yki missense mutation that abrogates Sd-Yki binding also inactivates Yki function in vivo. We further demonstrate that sd is required for yki-induced tissue overgrowth and target gene expression, and that sd activity is conserved in its mammalian homolog. Our results uncover a heretofore missing link in the Hpo signaling pathway and provide a glimpse of the molecular events on a Hpo-responsive enhancer element.",
author = "Shian Wu and Y. Liu and Yonggang Zheng and Jixin Dong and Duojia Pan",
year = "2008",
month = "1",
day = "1",
doi = "10.1016/j.devcel.2008.01.007",
language = "English (US)",
volume = "14",
pages = "388--398",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - The TEAD/TEF family protein Scalloped mediates transcriptional output of the Hippo growth-regulatory pathway.

AU - Wu, Shian

AU - Liu, Y.

AU - Zheng, Yonggang

AU - Dong, Jixin

AU - Pan, Duojia

PY - 2008/1/1

Y1 - 2008/1/1

N2 - The Hippo (Hpo) kinase cascade restricts tissue growth by inactivating the transcriptional coactivator Yorkie (Yki), which regulates the expression of target genes such as the cell death inhibitor diap1 by unknown mechanisms. Here we identify the TEAD/TEF family protein Scalloped (Sd) as a DNA-binding transcription factor that partners with Yki to mediate the transcriptional output of the Hpo growth-regulatory pathway. The diap1 (th) locus harbors a minimal Sd-binding Hpo Responsive Element (HRE) that mediates transcriptional regulation by the Hpo pathway. Sd binds directly to Yki, and a Yki missense mutation that abrogates Sd-Yki binding also inactivates Yki function in vivo. We further demonstrate that sd is required for yki-induced tissue overgrowth and target gene expression, and that sd activity is conserved in its mammalian homolog. Our results uncover a heretofore missing link in the Hpo signaling pathway and provide a glimpse of the molecular events on a Hpo-responsive enhancer element.

AB - The Hippo (Hpo) kinase cascade restricts tissue growth by inactivating the transcriptional coactivator Yorkie (Yki), which regulates the expression of target genes such as the cell death inhibitor diap1 by unknown mechanisms. Here we identify the TEAD/TEF family protein Scalloped (Sd) as a DNA-binding transcription factor that partners with Yki to mediate the transcriptional output of the Hpo growth-regulatory pathway. The diap1 (th) locus harbors a minimal Sd-binding Hpo Responsive Element (HRE) that mediates transcriptional regulation by the Hpo pathway. Sd binds directly to Yki, and a Yki missense mutation that abrogates Sd-Yki binding also inactivates Yki function in vivo. We further demonstrate that sd is required for yki-induced tissue overgrowth and target gene expression, and that sd activity is conserved in its mammalian homolog. Our results uncover a heretofore missing link in the Hpo signaling pathway and provide a glimpse of the molecular events on a Hpo-responsive enhancer element.

UR - http://www.scopus.com/inward/record.url?scp=42049116586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42049116586&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2008.01.007

DO - 10.1016/j.devcel.2008.01.007

M3 - Article

C2 - 18258486

AN - SCOPUS:42049116586

VL - 14

SP - 388

EP - 398

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 3

ER -